Assessing the combinatorial cytotoxicity of the exogenous contamination with BDE-209, bisphenol A, and acrylamide via high-content analysis
文献类型： 外文期刊
作者： Yuan, Jinwen 1 ; Che, Siyan 1 ; Zhang, Li 1 ; Li, Xiaomin 3 ; Yang, Junhua 4 ; Sun, Xiaoming 1 ; Ruan, Zheng 1 ;
作者机构： 1.Nanchang Univ, Inst Nutr, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China
2.Nanchang Univ, Sch Food Sci, Nanchang, Jiangxi, Peoples R China
3.Chinese Acad Agr Sci CAAS, Inst Qual Stand & Testing Technol Agroprod, Beijing, Peoples R China
4.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai, Peoples R China
关键词： Exogenous contaminations; Combinatorial cytotoxicity; High-content analysis; Chou-Talalay method; Synergistic effects
期刊名称：CHEMOSPHERE （ 影响因子：7.086； 五年影响因子：6.956 ）
ISSN： 0045-6535
年卷期： 2021 年 284 卷
页码：
收录情况： SCI
摘要： Food is often exposed to multiple types of contaminants, and the coexistence of contaminants may have antagonistic, additive or synergistic effects. This study investigated the combinatorial toxicity of the three most widespread exogenous contaminants, decabrominated diphenyl ether (BDE-209), bisphenol A (BPA), and acrylamide (ACR) to HepG2 cells. A mathematical model (Chou-Talalay) and high-content analysis (HCA) were used to probe the nature of the contaminants' interactions and their cytotoxicity mechanisms, respectively. The results highlighted that for the individual pollutants, the cytotoxicity order was BDE-209> BPA > ACR, and varying combinations of contaminants exhibited additive/synergistic effects. In general, combining multiple contaminants significantly increased intracellular reactive oxygen species (ROS), Ca2+ flux, DNA damage and Caspase-3, and decreased mitochondrial membrane potential (MMP) and nucleus roundness, indicating that the additive or synergistic mechanism of the combined contaminations was disturbance to multiple organelles. This study emphasizes the complexity of human exposure to food contaminants and provides a scientific basis for formulating strict regulatory standards.
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