文献类型： 外文期刊

作者： Liu, Tiansheng ¹ ; Zhan, Xue ¹ ; Li, Jinyu ¹ ; Ke, Fushi ⁴ ; Zhu, Xiangyu ¹ ; Chen, Yanting ¹ ; He, Weiyi ¹ ; You, Shijun ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian Taiwan Cro, Fuzhou, Peoples R China

2.Fujian Agr & Forestry Univ, Inst Appl Ecol, Fuzhou, Peoples R China

3.Guangdong Acad Agr Sci, Inst Fruit Tree Res,Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Key Lab Trop & Subtrop Fruit Tree Res, Guangzhou, Peoples R China

4.Univ Hong Kong, Sch Biol Sci, Hong Kong, Peoples R China

关键词： Microsatellite markers; Invasive species; Population genetics; Polymorphism; Cross-species amplification

期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY （ 影响因子：1.504； 五年影响因子：1.365 ）

ISSN： 0003-6862

年卷期： 2022 年 57 卷 2 期

页码：

收录情况： SCI

摘要： Liriomyza sativae is a globally distributed quarantine pest. We isolated polymorphic microsatellite loci from transcriptomic data for this species and tested their potential application in population genetics and cross-species transferability in other Agromyzidae species. Of the 130 microsatellite loci selected for validation, 40 were successfully amplified, and 18 were verified as polymorphic in L. sativae. The number of alleles (Na) ranged from two to ten, with polymorphism information content (PIC) values between 0.289 and 0.818. Observed (H-O) and expected (H-E) heterozygosity varied from 0.250 to 0.900 and 0.314 to 0.859, respectively. Low among-population differentiation was also indicated by pairwise F-ST (0.032-0.045). Structure analysis and principal coordinate analysis (PCoA) suggested three clusters for the 94 samples from three different locations (FZNT, SMSX, and QZYC) in Fujian Province, China. The low genetic differentiation and clear genetic structure among populations of this invasive species may result from extensive human activity. In additional, cross-species tests validated the amplification of 16, 19, 11 and 9 of 40 loci in Liriomyza huidobrensis, Liriomyza trifolii, Chromatomyia horticola and Ophiomyia phaseoli, respectively, showed the potential application of these markers for population genetic analysis in these species.