Isolation of a novel adomavirus from cultured American eels, Anguilla rostrata, with haemorrhagic gill necrosis disease
文献类型： 外文期刊
作者： Yang, Jin-Xian 1 ; Chen, Xi 1 ; Li, Ying-Ying 1 ; Song, Tie-Ying 1 ; Ge, Jun-Qing 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Biotechnol, Wusi Rd 247, Fuzhou 350003, Fujian, Peoples R China
关键词： adomavirus; Anguilla rostrata; haemorrhagic gill necrosis disease
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期： 2021 年 44 卷 11 期
页码：
收录情况： SCI
摘要： Recently, the culture of American eels (Anguilla rostrate) in China has been impacted by emergence of a disease with signs of haemorrhagic gill necrosis. The gills of diseased eels are covered with petecchia and they bleed when the operculum is pressed. In this study, a novel American eel adomavirus (AEAdoV) was isolated from the diseased eels using the eel ovary cell line (EO). The virus proliferated in the EO cells with a maximum TCID50/ml of 10(6.29 +/- 0.23) at 6 days post-infection. The virions were non-enveloped with a diameter of 75-85 nm and shown to be a DNA virus upon 5-iodo-2'-deoxyuridine (IDU) treatment. PCR assays showed that AEAdoV encodes a superfamily 3 helicases (S3H) replicase and shared high similarities with Anguilla marmorata adomavirus (MEAdoV). Although no clinical signs or mortality was observed among the eels injected with AEAdoV, the virus was reisolated from livers, kidneys and gills of injected eels at 35 days post-injection. Our results suggested that AEAdoV exhibited a latent infection in A. rostrata. The pathogenicity of the AEAdoV needs to be confirmed further.
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