文献类型： 外文期刊

作者： Xu, Yuan ¹ ; Du, Zhiheng ¹ ; Liu, Jiayu ¹ ; Su, Hang ¹ ; Ning, Fangyong ¹ ; Cui, Shiquan ¹ ; Wang, Lijuan ³ ; Liu, Jianmi ¹ ;

作者机构： 1.Northeast Agr Univ, Coll Anim Sci & Technol, 600 Changjiang Rd, Harbin 150030, Peoples R China

2.Northeast Agr Univ, Coll Life Sci, Harbin, Peoples R China

3.Eastern Liaoning Univ, Coll Agr, Dandong, Peoples R China

4.Yili Anim Sci Res Inst, Yining, Peoples R China

5.Anim Husb Adm Huadian, Huadian, Peoples R China

6.Branch Anim Husb & Vet, Qiqihar, Peoples R China

7.Heilongjiang Acad Agr Sci, Qiqihar, Peoples R China

关键词： male-linked molecular markers; Rana dybowskii; sexual reversal; TRAP markers

期刊名称：INTEGRATIVE ZOOLOGY （ 影响因子：2.654； 五年影响因子：2.525 ）

ISSN： 1749-4877

年卷期：

页码：

收录情况： SCI

摘要： Identifying the mechanism for sex determination in amphibians is challenging. Very little is known about sex determination mechanisms of Rana dybowskii, a species of importance to evolutionary and conservation biology. We screened for sex-linked molecular markers in R. dybowskii in China using target region amplification polymorphism with 2 fixed primers against the sequences of Dmrt1. We found 2 male-linked molecular markers in R. dybowskii, which were 222 bp and 261 bp long. The detection rates of 222 bp marker in males form Xinglong, Huadian, and Dandong were 93.79%, 69.64%, and 13.64%, respectively, while the rate in females from Huadian was 27.50%. Besides, the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%, 87.50%, and 32.73%, respectively. The inheritance patterns of sex-linked molecular markers showed that the 2 sex-linked molecular markers were heterozygous. Compared to the XY-male parent, progeny from XX-pseudo-male parent possessed lower sex reversal ratio at the same rearing temperature, and the proportion of female froglets from an XX-pseudo-male parent was more than 95% at low rearing temperature (15 degrees C). Our findings suggest that R. dybowskii displays male heterogamety, and the 2 sex-linked molecular markers may have a guiding significance for the protection and utilization of R. dybowskii.