RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly, Zeugodacus cucurbitae
文献类型： 外文期刊
作者： Ahmad, Shakil 1 ; Jamil, Momana 1 ; Fahim, Muhammad 2 ; Zhang, Shujing 1 ; Ullah, Farman 3 ; Lyu, Baoqian 4 ; Luo, Yan 1 ;
作者机构： 1.Hainan Univ, Sch Plant Protect, Haikou, Hainan, Peoples R China
2.Islamia Coll Univ, Ctr Om Sci, Peshawar, Pakistan
3.China Agr Univ, Coll Plant Protect, Dept Entomol, Beijing, Peoples R China
4.Chinese Acad Trop Agr Sci, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China
关键词： chitinase; mortality; RNA interference; wings malformation; Tephritidae
期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： This study reports the first successful use of oral feeding dsRNA technique for functional characterization of imaginal disc growth factors (IDGFs) genes (IDGF1, IDGF3_1, IDGF4_0, IDGF4_1, and IDGF6) in melon fly Zeugodacus cucurbitae. Phylogenetic and domain analysis indicates that these genes had high similarity with other Tephritidae fruit flies homolog and contain only one conserved domain among these five genes, which is glyco-18 domain (glyco-hydro-18 domain). Gene expression analysis at different developmental stages revealed that these genes were expressed at larval, pupal, and adult stages. To understand their role in different developmental stages, larvae were fed dsRNA-corresponding to each of the five IDGFs, in an artificial diet. RNAi-mediated knockdown of IDGF1 shows no phenotypic effects but caused mortality (10.4%), while IDGF4_0 caused malformed pharate at the adult stage where insects failed to shed their old cuticle and remained attached with their body, highest mortality (49.2%) was recorded compared to dsRNA-green fluorescent protein (GFP) or DEPC. Silencing of IDGF3_1 and IDGF4_1 cause lethal phenotype in larvae, (17.2%) and (40%) mortality was indexed in Z. cucurbitae. IDGF6 was mainly expressed in pupae and adult stages, and its silencing caused a malformation in adult wings. The developmental defects such as malformation in wings, larval-larval lethality, pupal-adult malformation, and small body size show that IDGFs are key developmental genes in the melon fly. Our results provide a baseline for the melon fly management and understanding of IDGFs specific functions in Z. cucurbitae.
- 相关文献
作者其他论文 更多>>
-
Seasonal migration pattern of an important rice pest, Nilaparvata lugens (Hemiptera: Delphacidae), on Hainan Island, China
作者：Gu, Fuxiong;Gu, Fuxiong;Lu, Hui;Lyu, Baoqian;Zhang, Qikai;Jiao, Bin;Tang, Jihong
关键词：brown planthopper; meteorological background; stable hydrogen isotope; seasonal cross-sea migration; trajectory
-
Performance and preference of larval parasitoid, Microplitis pallidipes (Hymenoptera: Braconidae), on 2 Spodoptera pest species
作者：Xu, Wei;Xu, Wei;Lyu, Baoqian;Yan, Sanqiang;Lyu, Baoqian;Song, Yaoyao;Tang, Jihong;Zhou, Xiaojuan;Wang, Chongtian
关键词：parasitoid; host selectivity; life table; pest control
-
Global scientific progress and shortfalls in biological control of the fall armyworm Spodoptera frugiperda
作者：Wyckhuys, Kris A. G.;Wyckhuys, Kris A. G.;Wang, Zhenying;Wu, Kongming;Wyckhuys, Kris A. G.;Akutse, Komivi S.;Calatayud, Paul-Andre;Dubois, Thomas;Khamis, Fathiya M.;Akutse, Komivi S.;Amalin, Divina M.;Araj, Salah-Eddin;Jaber, Lara R.;Barrera, Gloria;Espinel, Carlos;Gomez, Juliana;Beltran, Marie Joy B.;Montecalvo, Melissa P.;Ben Fekih, Ibtissem;Cokola, Marcellin C.;Dessauvages, Kenza;Francis, Frederic;Calatayud, Paul-Andre;Cicero, Lizette;Colmenarez, Yelitza C.;Durocher-Granger, Lena;Fallet, Patrick;Turlings, Ted C. J.;Fernandez-Triana, Jose L.;O'Hara, James E.;Haddi, Khalid;Silveira, Luis C.;Harrison, Rhett D.;Haseeb, Muhammad;Robinson-Baker, Ann M.;Simeon, Larisner;Iwanicki, Natasha S. A.;Wengrat, Ana P. G. S.;Legaspi, Jesusa C.;Lomeli-Flores, Refugio J.;Lopes, Rogerio B.;Lyu, Baoqian;Montoya-Lerma, James;Polaszek, Andrew;Nguyen, Tung D.;Nurkomar, Ihsan;Perier, Jermaine D.;Ramirez-Romero, Ricardo;Sanchez-Garcia, Francisco J.;Solter, Leellen F.;Santos-Amaya, Oscar F.;Talamas, Elijah J.;Tavares, Wagner de Souza;Trabanino, Rogelio;Valicente, Fernando H.;Vasquez, Carlos;Zang, Lian-Sheng;Zhang, Wei;Zimba, Kennedy J.;Elkahky, Maged;Hadi, Buyung A. R.;Hadi, Buyung A. R.
关键词：Ecological intensification; Global change; Biological control; Agroecology; Ecosystem services; Agrochemical pollution; Interdisciplinary science; Systems thinking; Invasive species management
-
Performance and population prediction of Opisina arenosella on four species of palm plants
作者：Yan, Sanqiang;Yan, Sanqiang;Lin, Nanfang;Kuang, Fuping;Lyu, Baoqian;Lu, Hui;Tang, Jihong;Zhang, Qikai;Qiu, Haiyan;Tang, Xue;Liu, Zhuo;Lyu, Baoqian;Lin, Nanfang;Xiang, Kaiping
关键词：Arecaceae; caterpillar; coconut; Cocos nucifera; Elaeis guineensis; Lepidoptera; life cycle; Livistona chinensis; Oecophoridae; palm plants; population size forecast; Roystonea regia
-
Ecology, invasion history and biodiversity-driven management of the coconut black-headed caterpillar Opisina arenosella in Asia
作者：Lu, Hui;Lyu, Baoqian;Tang, Jihong;Wu, Qiqi;Qiu, Haiyan;Zhang, Qikai;Lu, Hui;Lyu, Baoqian;Tang, Jihong;Wu, Qiqi;Qiu, Haiyan;Zhang, Qikai;Wyckhuys, Kris A. G.;Wyckhuys, Kris A. G.;Wyckhuys, Kris A. G.;Le, Khac Hoang;Chongchitmate, Patchareewan
关键词：coconut black-headed caterpillar; invasion biology; agroecology; crop protection; natural enemies; biological control; ecological intensification
-
Biological pest control protects pollinators
作者：Lyu, Baoqian;Wang, Shuchang;Liu, Zhuo;Wyckhuys, Kris A. G.;Wyckhuys, Kris A. G.;Wyckhuys, Kris A. G.
关键词：
-
Knockdown of the ecdysone receptor disrupts development and causes mortality in the melon fly, Zeugodacus cucurbitae
作者：Ahmad, Shakil;Jamil, Momana;Wu, Yuejie;Luo, Yanping;Jaworski, Coline C.;Palma-Onetto, Valeria;Lyu, Baoqian;Luo, Yanping
关键词：ecdysone receptor; gene silencing; lethal and sublethal effects; oral delivery; RNA interference
