Ethylene Response Factor EjERF23 From Loquat Promotes Cold Tolerance via Directly Regulating EjPOD Gene Participated in Scavenging of Hydrogen Peroxide
文献类型： 外文期刊
作者： Zhang, Jiaying 1 ; An, Haishan 1 ; Li, Shuigen 1 ; Zhou, Boqiang 1 ; Zhang, Xueying 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Protected Hort Technol, Shanghai, Peoples R China
关键词： cold stress; ERF transcription factor; loquat; peroxidase
期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.3； 五年影响因子：7.7 ）
ISSN： 0140-7791
年卷期： 2025 年
页码：
收录情况： SCI
摘要： In higher plants, ethylene response factors (ERFs) play crucial roles in orchestrating cold stress signal transduction. This study validated EjERF23 in loquat (Eriobotrya japonica Lindl.) and elucidated its role in cold tolerance. The transcriptional expression of EjERF23 was augmented upon exposure to the ethylene precursor 1-aminocyclopropane-1-carboxylate and low temperatures. Concurrently, it was inhibited by the ethylene inhibitor aminoethoxyvinylglycine under cold conditions. Overexpression of EjERF23 in transgenic tobacco enhanced cold tolerance, exhibiting significantly increased peroxidase (POD) activity, leading to reduced hydrogen peroxide (H2O2) accumulation and improved antioxidant stress tolerance. Further analysis revealed that EjERF23 directly interacts with the EjPOD47 promoter, increasing its transcriptional level. The outcomes of the yeast expression assay indicated that EjPOD47 has notable functions in the response to cold stress. Tobacco lines that were genetically modified and treated with POD inhibitors exhibited elevated content of H2O2 and a remarkable decrease in cold tolerance. The findings indicated that the EjERF23 protein is crucial for enhancing cold tolerance by positively regulating genes that code for POD to eliminate reactive oxygen species. Our findings offer fresh perspectives on the molecular regulatory mechanisms that govern the ethylene signaling pathway related to responses to cold stress.
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