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Genome-wide identification and expression analysis of the bHLH gene family in cauliflower (Brassica oleracea L.)

文献类型： 外文期刊

作者： Jiang, Hanmin 1 ; Liu, Lili 1 ; Shan, Xiaozheng 1 ; Wen, Zhenghua 2 ; Zhang, Xiaoli 2 ; Yao, Xingwei 1 ; Niu, Guobao 2 ; Shan, Changliang 5 ; Sun, Deling 1 ;

作者机构： 1.Tianjin Acad Agr Sci, Tianjin 300192, Peoples R China

2.Tianjin Kernel Agr Technol Co Ltd, Vegetable Res Inst, Tianjin 300384, Peoples R China

3.State Key Lab Vegetable Germplasm Innovat, Tianjin 300384, Peoples R China

4.Tianjin Key Lab Vegetable Genet & Breeding, Tianjin 300384, Peoples R China

5.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300350, Peoples R China

6.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300350, Peoples R China

关键词： Cauliflower; bHLH gene family; Bioinformatics analysis; Expression analysis

期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 影响因子：3.023； 五年影响因子：3.442 ）

ISSN： 0971-5894

年卷期： 2022 年 28 卷 9 期

页码：

收录情况： SCI

摘要： Basic helix-loop-helix (bHLH) transcription factors (TFs) are one of the largest TF families in plant species, and they play important roles in plant growth, development and stress responses. The present study systematically identified members of the cauliflower (Brassica oleracea L.) bHLH gene family based on genomic data. Analysis of bHLH family gene numbers, evolution, collinearity, gene structures and motifs indicated that cauliflower contained 256 bHLH family genes distributed on 10 chromosomes. Most of these genes have been localized in the nucleus, and they were divided into 18 subgroups which have been relatively conserved during evolution. Promoter analysis showed that most cis-acting elements were related to MeJA and ABA. Expression analysis suggested that 14 bHLH genes may be involved in the transformation of cauliflower curd from white to purple. An expression analysis of these 14 genes in FQ136 material was performed using qRT-PCR, and 9 bHLH genes (BobHLH1, 14, 58, 61, 63, 84, 231, 239 and 243) showed significantly increased or decreased expression in cauliflower from white to purple, which suggests that these 9 genes play important roles in the accumulation of anthocyanins in cauliflower. The coexpression network of these 9 genes and anthocyanin synthesis-related key genes was analyzed using weighted gene coexpression network analysis (WGCNA). In conclusion, our observations suggested that the bHLH gene family plays an important role in the accumulation of anthocyanins in cauliflower and provide an important theoretical basis for further research on the functions of the bHLH gene family and the molecular mechanism of cauliflower coloration.

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