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Response of Fusarium pseudograminearum to Biocontrol Agent Bacillus velezensis YB-185 by Phenotypic and Transcriptome Analysis

文献类型： 外文期刊

作者： Zhang, Jie 1 ; Zhu, Wenqian 1 ; Goodwin, Paul H. 2 ; Lin, Qitong 1 ; Xia, Mingcong 1 ; Xu, Wen 1 ; Sun, Runhong 1 ; Liang, Juan 1 ; Wu, Chao 1 ; Li, Honglian 3 ; Wang, Qi 4 ; Yang, Lirong 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Biopesticide Engn Res Ctr, Henan Agr Microbiol Innovat Ctr, Inst Plant Protect Res, Zhengzhou 450002, Peoples R China

2.Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

3.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China

4.China Agr Univ, Coll Plant Protect, Dept Plant Pathol, Beijing 100193, Peoples R China

关键词： Fusarium crown rot; Bacillus velezensis; biocontrol; RNA-seq

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

ISSN：

年卷期： 2022 年 8 卷 8 期

页码：

收录情况： SCI

摘要： The use of biological control agents (BCAs) is a promising alternative control measure for Fusarium crown rot (FCR) of wheat caused by Fusarium pseudograminearum. A bacterial strain, YB-185, was isolated from the soil of wheat plants with FCR and identified as Bacillus velezensis. YB-185 exhibited strong inhibition of F. pseudograminearum mycelial growth and conidial germination in culture. Seed treatment with YB-185 in greenhouse and field resulted in reductions in disease by 66.1% and 57.6%, respectively, along with increased grain yield. Microscopy of infected root tissues confirmed that YB-185 reduced root invasion by F. pseudograminearum. RNA-seq of F. pseudograminearum during co-cultivation with B. velezensis YB-185 revealed 5086 differentially expressed genes (DEGs) compared to the control. Down-regulated DEGs included genes for glucan synthesis, fatty acid synthesis, mechanosensitive ion channels, superoxide dismutase, peroxiredoxin, thioredoxin, and plant-cell-wall-degrading enzymes, whereas up-regulated DEGs included genes for chitin synthesis, ergosterol synthesis, glutathione S-transferase, catalase, and ABC transporters. In addition, fungal cell apoptosis increased significantly, as indicated by TUNEL staining, and the scavenging rate of 2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS center dot+) in the fungus significantly decreased. Thus, F. pseudograminearum may be trying to maintain normal cell functions by increasing cell wall and membrane synthesis, antioxidant and anti-stress responses, detoxification of bacterial antimicrobial compounds, and transportation of damaging compounds from its cells. However, cell death and free radical accumulation still occurred, indicating that the responses were insufficient to prevent cell damage. Bacillus velezensis YB-185 is a promising BCA against FCR that acts by directly damaging F. pseudograminearum, thus reducing its ability to colonize roots and produce symptoms.

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