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Functional characterization of TBL genes revealed the role of GhTBL7 and GhTBL58 in cotton fiber elongation

文献类型： 外文期刊

作者： Kabir, Nosheen 1 ; Wang, Xuwen 3 ; Lu, Lili 2 ; Qanmber, Ghulam 1 ; Liu, Le 2 ; Si, Aijun 3 ; Zhang, Lian 3 ; Cao, Wei 3 ; Yang, Zuoren 3 ; Yu, Yu 3 ; Liu, Zhao 1 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou 450001, Henan, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, Cotton Res Inst, Key Lab China Northwestern Inland Reg, Minist Agr & Rural Affairs, Shihezi 832003, Peoples R China

4.Chinese Acad Agr Sci, Western Agr Res Ctr, Changji 831100, Peoples R China

关键词： G; hirsutum; TBL; Fiber development; Phylogenetic analysis; Gene silencing

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 241 卷

页码：

收录情况： SCI

摘要： TBL (Trichome Birefringence Like) gene family members are involved in trichome initiation and xylan acetylation in several plant species. In our research, we identified 102 TBLs from G. hirsutum. The phylogenetic tree classified TBL genes into five groups. Collinearity analysis of TBL genes indicated 136 paralogous gene pairs in G. hirsutum. Gene duplication indicated that WGD or segmental duplication contributed to the GhTBL gene family expansion. Promoter cis-elements of GhTBLs were related to growth and development, seed-specific regulation, light, and stress responses. GhTBL genes (GhTBL7, GhTBL15, GhTBL21, GhTBL25, GhTBL45, GhTBL54, GhTBL67, GhTBL72, and GhTBL77) exhibited upregulated response under exposure to cold, heat, NaCl, and PEG. GhTBL genes exhibited high expression during fiber development stages. Two GhTBL genes (GhTBL7 and GhTBL58) showed differential expression at 10 DPA fiber, as 10 DPA is a fast fiber elongation stage and fiber elongation is a very important stage of cotton fiber development. Subcellular localization of GhTBL7 and GhTBL58 revealed that these genes reside inside the cell membrane. Promoter GUS activity of GhTBL7 and GhTBL58 exhibited deep staining in roots. To further validate the role of these genes in cotton fiber elongation, we silenced these genes and observed a significant reduction in the fiber length at 10 DPA. In conclusion, the functional study of cell membrane-associated genes (GhTBL7 and GhTBL58) showed deep staining in root tissues and potential function during cotton fiber elongation at 10 DPA fiber.

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