Fermentation Regulation: Revealing Bacterial Community Structure, Symbiotic Networks to Function and Pathogenic Risk in Corn Stover Silage
文献类型： 外文期刊
作者： Du, Zhumei 1 ; Cui, Shaojuan 1 ; Chen, Yifan 1 ; Zhang, Yunhua 1 ; Wang, Siran 2 ; Yan, Xuebing 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
关键词： crop by-product; microbial co-occurrence network; pathogen assessment; regulation mechanisms; silage function prediction
期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.8 ）
ISSN：
年卷期： 2025 年 15 卷 16 期
页码：
收录情况： SCI
摘要： Improving agricultural by-product utilization can alleviate tropical feed shortages. This study used corn stover (CS, Zea mays L.) at the maturity stage as the material, with four silage treatments: control, lactic acid bacteria (LAB, Lactiplantibacillus plantarum), cellulase (AC, Acremonium cellulolyticus), and LAB+AC. After 60 days fermentation in plastic drum silos, the silos were opened for sampling. PacBio single-molecule real-time sequencing technology was used to study bacterial community structure, symbiotic network functionality, and pathogenic risk to clarify CS fermentation regulatory mechanisms. The CS contained 59.9% neutral detergent fiber and 7.1% crude protein. Additive-treated silages showed better quality than the control: higher lactic acid (1.64-1.83% dry matter, DM), lower pH (3.62-3.82), and reduced ammonia nitrogen (0.54-0.81% DM). Before ensiling, the CS was dominated by Gram-negative Rhizobium larrymoorei (16.30% of the total bacterial community). Functional prediction indicated that the microbial metabolism activity in diverse environments was strong, and the proportion of potential pathogens was relatively high (14.69%). After ensiling, Lactiplantibacillus plantarum as Gram-positive bacteria were the dominant species in all the silages (58.39-84.34% of the total bacterial community). Microbial additives facilitated the establishment of a symbiotic microbial network, where Lactiplantibacillus occupied a dominant position (p < 0.01). In addition, functional predictions showed an increase in the activity of the starch and sucrose metabolism and a decrease in the proportion of potential pathogens (0.61-1.95%). Among them, the synergistic effect of LAB and AC inoculants optimized the silage effect of CS. This study confirmed that CS is a potential high-quality roughage resource, and the application of silage technology can provide a scientific basis for the efficient utilization of feed resources and the stable development of animal husbandry in the tropics.
- 相关文献
作者其他论文 更多>>
-
Biological delignification and anaerobic fermentation of wheat straw: A promising approach for sustainable utilization of crop straw bioresources
作者：Nazar, Mudasir;Tian, Jipeng;Wang, Xin;Wang, Siran;Cheng, Yunhui;Zhang, Wenjie;Xu, Nengxiang;Liu, Beiyi;Ding, Chenglong;Nazar, Mudasir;Tian, Jipeng;Wang, Xin;Wang, Siran;Cheng, Yunhui;Zhang, Wenjie;Xu, Nengxiang;Liu, Beiyi;Ding, Chenglong;Khan, Nazir Ahmad
关键词：Crop residues; Wheat straw; Lignin degradation; Anaerobic fermentation; Enzymatic hydrolysis; In vitro digestibility
-
Assessment of Effects of Storage Time on Fermentation Profile, Chemical Composition, Bacterial Community Structure, Co-Occurrence Network, and Pathogenic Risk in Corn Stover Silage
作者：Du, Zhumei;Meng, Ying;Chen, Yifan;Cui, Shaojuan;Yan, Xuebing;Wang, Siran
关键词：bacterial community structure; corn stover; crop by-product; microbial network system; silage fermentation dynamics
-
Evaluation of growth stage and storage time on fermentation characteristics, microbial community structure, co-occurrence networks, and their functional shifts and pathogenic risk of fermented Italian ryegrass
作者：Wang, Siran;Ding, Chenglong;Tian, Jipeng;Cheng, Yunhui;Xu, Nengxiang;Zhang, Wenjie;Wang, Xin;Nazar, Mudasir;Liu, Beiyi;Wang, Siran;Ding, Chenglong;Tian, Jipeng;Cheng, Yunhui;Xu, Nengxiang;Zhang, Wenjie;Wang, Xin;Nazar, Mudasir;Liu, Beiyi
关键词：Growth stage; Fermentation product; Microbial community; Lolium multiflorum; Microbial risk
-
Effect of biological lignin depolymerization on rice straw enzymatic hydrolysis, anerobic fermentation characteristics and in vitro ruminal digestibility
作者：Nazar, Mudasir;Tian, Jipeng;Wang, Xin;Wang, Siran;Cheng, Yunhui;Zhang, Wenjie;Xu, Nengxiang;Liu, Beiyi;Ding, Chenglong;Nazar, Mudasir;Tian, Jipeng;Wang, Xin;Wang, Siran;Cheng, Yunhui;Zhang, Wenjie;Xu, Nengxiang;Liu, Beiyi;Ding, Chenglong;Khan, Nazir Ahmad
关键词：Rice straw; Lignin depolymerization; Ensiling; Enzymatic hydrolysis; In vitro digestibility
-
Using null models to decipher bacterial assembly mechanisms in oat silages harvested from southern China
作者：Dong, Zhihao;Hu, Shiwei;Zhao, Jie;Wang, Siran;Li, Junfeng;Shao, Tao;Fang, Di
关键词：Bacterial assembly; Null models; Oat silage; Southern China
-
Staurosporine-producing Streptomyces sp. strain 11 x 1 cell-free culture filtrates control diseases caused by the oomycete plant pathogens Pythium myriotylum and Phytophthora sojae
作者：Wen, Xian;Wang, Lunji;Li, Jingjing;Jiu, Min;Wen, Xian;Li, Jingjing;Chen, Yifan;Zhuo, Deyu;Anjago, Wilfred Mabeche;Zhu, Hongli;Zhang, Qimeng;Zhang, Jinfeng;Wang, Xiaoyu;Wei, Lihui;Daly, Paul;Zhou, Dongmei;Chen, Yifan;Wei, Lihui;Peng, Hao;Wang, Lunji;Daly, Paul;Zhou, Dongmei
关键词：Streptomyces; Pythium myriotylum; Phytophthora sojae; Biological control; Ginger; Soybean
-
An evaluation of storage length on ensiling characteristics, bacterial community compositions, co-occurrence networks, and their functional shifts and pathogenic risk in high-moisture oat silage
作者：Wang, Siran;Ding, Chenglong;Tian, Jipeng;Cheng, Yunhui;Xu, Nengxiang;Zhang, Wenjie;Wang, Xin;Nazar, Mudasir;Liu, Beiyi;Wang, Siran;Ding, Chenglong;Tian, Jipeng;Cheng, Yunhui;Xu, Nengxiang;Zhang, Wenjie;Wang, Xin;Nazar, Mudasir;Liu, Beiyi
关键词：Oat; Ensiling; Silage; Fermentation quality; Bacterial community
