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TaPR1 Interacts With TaTLP1 via the aIV Helix to Be Involved in Wheat Defense to Puccinia triticina Through the CAPE1 Motif

文献类型： 外文期刊

作者： Wang, Fei 1 ; Shen, Songsong 1 ; Zhao, Cunpeng 1 ; Cui, Zhongchi 1 ; Meng, Linshuo 1 ; Wu, Wenyue 1 ; Liu, Daqun 1 ; Wang, Haiyan 1 ;

作者机构： 1.Hebei Agr Univ, Coll Plant Protect, Technol Innovat Ctr Biol Control Crop Dis & Insect, Baoding, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Cotton, Shijiazhuang, Peoples R China

关键词： TaPR1-4; alpha IV helix; CAPE1; resistance mechanisms; Triticum aestivum; Puccinia triticina

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Pathogenesis-related (PR) proteins play important roles in plant defense response and systemic acquired resistance (SAR). PR1 has antifungal activity against many plant pathogens. In our previous study, RNA sequencing (RNA-seq) was conducted on resistant wheat line TcLr19 and sensitive wheat cultivar Chinese Spring inoculated with Puccinia triticina (Pt) race PHNT. In this study, seven salicylic acid (SA)-induced TaPR1 genes involved in plant disease resistance were found in the RNA-seq library. Quantitative PCR (qPCR) results showed that TaPR1-4 was most induced by Pt among these seven TaPR1 genes in the incompatible interaction. Yeast two-hybrid (Y2H) results showed that TaPR1-4 interacted with TaTLP1 via the alpha IV helix. Protein-mediated phenotyping assays in vivo and antifungal activity in vitro demonstrated that wheat leaves infiltrated with pure TaPR1-4 protein developed significantly less disease compared to control leaves. This effect was correlated with a strong increase in defense gene expression, and resistance activity was dependent on the CAPE1 motif located in the C-terminal region of TaPR1-4. These findings increase current knowledge regarding the interaction of TaPR1 and TaTLP1 and provide new insights on the role of TaPR1 protein in the resistance of wheat to Pt.

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