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Interleukin-6 in Siberian sturgeon (Acipenser baeri): Molecular characterization and immune functional activity

文献类型： 外文期刊

作者： Wang, Xiaowen 1 ; Chen, Jingyi 1 ; Zhang, Rong 1 ; Liu, Lili 1 ; Ma, Guoqing 1 ; Zhu, Hua 1 ;

作者机构： 1.Beijing Fisheries Res Inst, Beijing 100068, Peoples R China

2.Beijing Key Lab Fishery Biotechnol, Beijing 100068, Peoples R China

3.Natl Freshwater Fisheries Engn Technol Res Ctr, Beijing 100068, Peoples R China

关键词： Interleukin-6 (IL-6); Siberian sturgeon (Acipenser baeri); Inflammatory response; Recombinant expression; Immunoregulation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 102 卷

页码：

收录情况： SCI

摘要： Interleukin-6 (IL-6) is a pleiotropic cytokine with crucial immunoregulatory functions in both innate and adaptive immune responses. However, the IL-6 sequence and function remain unknown in sturgeon, one chondrostean fish. In the present study, we identified an interleukin-6 homolog from Siberian sturgeon (Acipenser bath), named AbIL-6. Its open reading frame (ORF) was 657 nucleotides in length, encoding a polypeptide of 218 amino acids, which contains a signal peptide and the IL-6 family domain. Phylogenetic analysis showed that sturgeon IL-6 had close relationship with both teleost and chondrichthyes IL-6s. Abil-6 mRNA was highly expressed in spleen, brain and liver tissues of healthy sturgeon, and significantly up-regulated in the spleen, head kidney and liver by A.hydrophila (A.h) challenge. Heat-killed A.h and LPS effectively stimulated Abil-6 transcripts in primary spleen cells in vitro. In order to understand the bioactivity and influence of AbIL-6 on immune responses, recombinant AbIL-6 (rAbIL-6) was synthesized by prokaryotes and demonstrated to promote the proliferation of spleen cells and head kidney cells in vitro. Additionally, intraperitoneal injection of rAbIL-6 induced significantly higher expression of four immuno-related genes including it-1 beta, cxcl10, mhcII beta and igm. rAbIL-6 improved the survival rate and reduced the tissue bacterial load after A.h infection. Taken together, these results suggest that AbIL-6 plays an important role in inflammatory responses and immune defense against bacterial infection of sturgeon.

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[1]Interleukin-6 in Siberian sturgeon (Acipenser baeri): Molecular characterization and immune functional activity. Wang, Xiaowen,Chen, Jingyi,Zhang, Rong,Liu, Lili,Ma, Guoqing,Zhu, Hua,Wang, Xiaowen,Chen, Jingyi,Zhang, Rong,Liu, Lili,Ma, Guoqing,Zhu, Hua,Wang, Xiaowen,Chen, Jingyi,Zhang, Rong,Liu, Lili,Ma, Guoqing,Zhu, Hua. 2020

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