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Advances of CRISPR/Cas-based Biosensor in Detection of Food-Borne Pathogens

文献类型： 外文期刊

作者： Zhang, Xiao-Yuan 1 ; Yao, Zhi-Hao 2 ; He, Kai-Yu 2 ; Wang, Hong-Mei 2 ; Xu, Xia-Hong 2 ; Wu, Zu-Fang 1 ; Wang, Liu 2 ;

作者机构： 1.Ningbo Univ, Coll Food & Pharm, Ningbo 315000, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou 310021, Peoples R China

关键词： Food-borne pathogens; CRISPR/Cas; Biosensor; Review

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.2； 五年影响因子：1.0 ）

ISSN： 0253-3820

年卷期： 2024 年 52 卷 4 期

页码：

收录情况： SCI

摘要： Rapid and accurate detection methods for food -borne pathogens are essential to ensure food safety and human health. One promising innovation in this area is the clustered regularly interspaced short palindromic repeats/CRISPR -associated systems (CRISPR/Cas) biosensor, which utilizes Cas protein and CRISPR RNA (crRNA) ribonucleo protein to specifically recognize target genes, and converts target signals into detectable physical and chemical signals. The CRISPR/Cas biosensor shows many advantages, such as high specificity, programmability, and ease of use, making it promising to pathogen detection. This paper introduced the principles and characteristics of CRISPR/Cas systems, along with the strategies for signal recognition, amplification, and output based on different CRISPR/Cas biosensors, and their respective applications in food -borne pathogen detection. Furthermore, the construction principles and challenges of multiple biosensors based on CRISPR/Cas were explored, as well as their potential for simultaneous detection of multiple pathogens. Finally, the challenges and future development trends of CRISPR/Cas -based biosensors in rapid pathogen detection were discussed, aiming to provide valuable reference and inspiration for biosensor designers and food safety practitioners.

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