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Label-Free Sequence-Specific Visualization of LAMP Amplified Salmonella via DNA Machine Produces G-Quadruplex DNAzyme

文献类型： 外文期刊

作者： Zeng, Huan 1 ; Huang, Shuqin 1 ; Chen, Yunong 1 ; Chen, Minshi 3 ; He, Kaiyu 2 ; Fu, Caili 1 ; Wang, Qiang 2 ; Zhang, Fang 1 ; Wang, Liu 2 ; Xu, Xiahong 2 ;

作者机构： 1.Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou 310021, Peoples R China

3.Technol Ctr Fuzhou Customs Dist, Fuzhou 350015, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Traceabil Agr Genet Modified Organisms, Hangzhou 310021, Peoples R China

关键词： loop-mediated isothermal amplification; colorimetric detection; sequence-specific; G-quadruplex; DNA machine

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN：

年卷期： 2023 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Salmonella is one of four key global causes of diarrhea, and in humans, it is generally contracted through the consumption of contaminated food. It is necessary to develop an accurate, simple, and rapid method to monitor Salmonella in the early phase. Herein, we developed a sequence-specific visualization method based on loop-mediated isothermal amplification (LAMP) for the detection of Salmonella in milk. With restriction endonuclease and nicking endonuclease, amplicons were produced into single-stranded triggers, which further promoted the generation of a G-quadruplex by a DNA machine. The G-quadruplex DNAzyme possesses peroxidase-like activity and catalyzes the color development of 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid) (ABTS) as the readouts. The feasibility for real samples analysis was also confirmed with Salmonella spiked milk, and the sensitivity was 800 CFU/mL when observed with the naked eye. Using this method, the detection of Salmonella in milk can be completed within 1.5 h. Without the involvement of any sophisticated instrument, this specific colorimetric method can be a useful tool in resource-limited areas.

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