文献类型： 外文期刊

作者： Guo, Lei ¹ ; Tang, Baozhen ¹ ; Dong, Wei ¹ ; Liang, Pei ¹ ; Gao, Xiwu ¹ ;

作者机构： 1.China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China

2.Chinese Acad Sci, Inner Mongolia Prataculture Res Ctr, Hohhot, Peoples R China

关键词： Plutella xylostella;ryanodine receptor;diamide insecticide;mRNA expression

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2012 年 68 卷 12 期

页码：

收录情况： SCI

摘要： BACKGROUND: The rynodine receptors (RyRs) are the main targets of diamide insecticides such as chlorantraniliprole. To provide the basis for a good understanding of the molecular mechanisms of diamide insecticide resistance, an RyR gene from Plutella xylostella was cloned and characterised in the present paper. RESULTS: A full-length cDNA sequence of RyR was cloned from P. xylostella through RT-PCR and rapid amplification of cDNA ends (RACE). The gene (named PxRyR1) is 15 753 bp long, with an open reading frame of 15 354 bp, encoding a predicted RyR of 5117 amino acids. An alternative splicing of the PxRyR1 was also cloned and named PxRyR2. The PxRyR1 shares 7793% identity with other insect RyRs. Quantitative real-time PCR analysis showed that the PxRyR was expressed at a high level in second-instar larvae and adults, at a low level in prepupae and pupae and abundantly in the body wall muscle and head (respectively 6.00 and 3.12 times the expression in the gut). Western blot analysis with anti-RyR antibodies showed that the RyR was mainly present in the body wall muscle and head, but barely present in the haemocyte and gut. CONCLUSIONS: There are at least two alternative splices of PxRyR expressed in all developmental stages and tissues in P. xylostella at various levels. The results provided the basis for further understanding of the mechanisms of resistance to diamide insecticides in P. xylostella. Copyright (C) 2012 Society of Chemical Industry