文献类型： 外文期刊

作者： Yuan Lin ¹ ; Lu Fuping ¹ ; Liu Shanshan ² ; Wang Xiao ² ; Zhang Ruixuan ² ; Li Ziqin ³ ; Zhang Hui ³ ;

作者机构： 1.Tianjin Univ Sci & Technol, Key Lab Ind Microbiol, Coll Biotechnol, Tianjin 300222, Peoples R China

2.Inner Mongolia Univ, Coll Life Sci, Hohhot 010021, Peoples R China

3.Inner Mongolia Acad Agr & Anim Husb, Hohhot 010021, Peoples R China

关键词： Fusarium oxysporum Schl.;ISSR;Reaction system optimization;Cluster analysis;Genetic diversity

期刊名称：ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 1

ISSN：

年卷期： 2011 年

页码：

收录情况： SCI

摘要： An Orthogonal design is applied to the optimization of ISSR-PCR reaction system for Fusarium oxysporum Schl. The effect of the five main factors was tested. A most suitable ISSR-PCR reaction system for Fusarium oxysporum Schl. was established. The optimized reaction system consists of 20 ng template DNA, 1 U Taq DNA polymerase, 0.4 mu mol/L primer, 0.2 mmol/L dNTPs, 4.0 mmol/L Mg2+ and 2.5 mu L10x buffer. Optimal annealing temperature was decided by different primers. 2 primers with stable amplification and rich polymorphism for ISSR were selected. Based on the optimizing, 30 isolated strains from Hebei were tested in order to analyse the genetic diversity between of Fusarium oxysporum Schl. The isolated 30 strains were clustered into 2 genetic line-ages at 0.86 genetic similarity. The genetic lineages of 30 strains showed no obvious relation with their geographical originals.