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Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant

文献类型： 外文期刊

作者： Han, Yike 1 ; Zhao, Fengyue 3 ; Gao, Shang; Wang, Xianyun; Wei, Aimin 2 ; Chen, Zhengwu 2 ; Liu, Nan 2 ; Tong, Xueq 1 ;

作者机构： 1.China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China

2.Tianjin Kernel Cucumber Res Inst, Tianjin Key Lab Vegetable Breeding Enterprise, State Key Lab Vegetable Germplasm Innovat, Tianjin 300192, Peoples R China

3.Nankai Univ, Coll

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2018 年 131 卷 2 期

页码：

收录情况： SCI

摘要： The cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an MMD1 -like gene Csa3M006660 that may be responsible for the male sterile in cucumber. A cucumber (Cucumis sativus L.) male sterile mutant (ms-3) in an advanced-generation inbred line was identified, and genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, ms-3, which was stably inherited. Histological studies suggested that the main cause of the male sterility was defective microsporogenesis, resulting in no tetrad or microspores being formed. Bulked segregant analysis (BSA) and genotyping of an F-2 population of 2553 individuals were employed used to fine map ms-3, which was delimited to a 76 Kb region. In this region, a single non-synonymous SNP was found in the Csa3M006660 gene locus, which was predicted to result in an amino acid change. Quantitative RT-PCR analysis of Csa3M006660 was consistent with the fact that it plays a role in the early development of cucumber pollen. The protein encoded by Csa3M006660 is predicted to be homeodomain (PHD) finger protein, and the high degree of sequence conservation with homologs from a range of plant species further suggested the importance of the ms-3 non-synonymous mutation. The data presented here provide support for Csa3M006660 as the most likely candidate gene for Ms-3.

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