Single-Cell RNA Sequencing of the Testis of Ciona intestinalis Reveals the Dynamic Transcriptional Profile of Spermatogenesis in Protochordates
文献类型： 外文期刊
作者： Li, Yanan 1 ; Liu, Xiang 1 ; Zhang, Xianghui 2 ; Wang, Hongyan 2 ; Chen, Jianyang 5 ; Wei, Jiankai 7 ; Li, Yubang 2 ; Chen, Hongxi 5 ; Wang, Qian 2 ; Liu, Kaiqiang 2 ; Liu, Yuyan 2 ; Shao, Changwei 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao 266072, Peoples R China
3.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian 116023, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
5.BGI, BGI Res Qingdao, Qingdao 266555, Peoples R China
6.Qingdao Europe Adv Inst Life Sci, BGI Qingdao, Qingdao 266555, Peoples R China
7.Ocean Univ China, Coll Marine Life Sci, Sars Fang Ctr, MoE Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China
8.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
关键词： spermatogenesis; scRNA-seq; Ciona intestinalis; protochordates
期刊名称：CELLS （ 影响因子：7.666； 五年影响因子：7.677 ）
ISSN：
年卷期： 2022 年 11 卷 24 期
页码：
收录情况： SCI
摘要： Spermatogenesis is a complex and continuous process of germ-cell differentiation. This complex process is regulated by many factors, of which gene regulation in spermatogenic cells plays a decisive role. Spermatogenesis has been widely studied in vertebrates, but little is known about spermatogenesis in protochordates. Here, for the first time, we performed single-cell RNA sequencing (scRNA-seq) on 6832 germ cells from the testis of adult Ciona intestinalis. We identified six germ cell populations and revealed dynamic gene expression as well as transcriptional regulation during spermatogenesis. In particular, we identified four spermatocyte subtypes and key genes involved in meiosis in C. intestinalis. There were remarkable similarities and differences in gene expression during spermatogenesis between C. intestinalis and two other vertebrates (Chinese tongue sole and human). We identified many spermatogenic-cell-specific genes with functions that need to be verified. These findings will help to further improve research on spermatogenesis in chordates.
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