A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops
文献类型： 外文期刊
作者： Zeng, Haijuan 1 ; Yang, Qianwen 1 ; Liu, Hua 1 ; Wu, Guogan 1 ; Jiang, Wei 1 ; Liu, Xiaofeng 3 ; Wang, Jinbin 1 ; Tang, X 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
2.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai 201106, Peoples R China
3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
关键词： Immunosensor; Gold nanoparticle-antibody; Graphene and PAMAM composites; Genetically modified crops
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2021 年 361 卷
页码：
收录情况： SCI
摘要： A simple electrochemical immunosensor based on nitrogen-doped graphene and polyamide-amine (GN-PAM) composites was proposed for the detection of the CP4-EPSPS protein in genetically modified (GM) crops. In this immunosensor, the amplification of the detection signal was realized through antibodies labeled with gold nanoparticles (AuNPs). The electrochemical responses of the immunosensor were linear (R-2 = 0.9935 and 0.9912) when the GM soybean RRS and maize NK603 content ranged from 0.025% to 1.0% and 0.05% to 1.5%, respectively. The limits of detection for the GM soybean RRS and maize NK603 were as low as 0.01% and 0.03%, respectively. The immunosensor also exhibited high specificity, and satisfactory stability, reproducibility, and accuracy. Our findings indicated that the constructed immunosensor provides a new approach for the sensitive detection of the CP4-EPSPS protein. Notably, the sensor may be applied to other proteins or pathogenic bacteria by simply changing the antibodies, and may also be used for multi-component analysis.
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