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A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection

文献类型: 外文期刊

作者: Luan, Yunxia 1 ; Lu, Anxiang 1 ; Chen, Jiayi 1 ; Fu, Hailong 1 ; Xu, Li 1 ;

作者机构: 1.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

2.Beijing Municipal Key Lab Agr Environm Monitoring, Beijing 100097, Peoples R China

关键词: aptamer;biosensor;cadmium;PicoGreen;fluorescence

期刊名称:APPLIED SCIENCES-BASEL ( 影响因子:2.679; 五年影响因子:2.736 )

ISSN: 2076-3417

年卷期: 2016 年 6 卷 12 期

页码:

收录情况: SCI

摘要: Selective detection of ultratrace amounts of cadmium (Cd2+) is extremely important for food safety and environmental monitoring because of its toxicity and widespread use. In this work, we developed a facile, rapid, sensitive, and highly selective method for the detection of Cd2+ based on a label-free aptasensor using an unmodified double-stranded deoxyribonucleic acid-specific dye (PicoGreen). The linear range was 0.10-100 mu g/mL, and the detection limit (0.038 ng/mL) was lower than the guideline from the World Health Organization for Cd2+ in drinking water (3 ng/mL). The sensor exhibited excellent selectivity towards Cd2+ ions. We tested the aptasensor in application to a series of real water samples spiked with different concentrations of Cd2+. Compared with atomic absorption spectrometry, the results showed good tolerance to the matrix effect. The developed approach shows great potential for on-site and high-throughput analysis in routine monitoring.

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