文献类型： 外文期刊

作者： Liu, Ya ¹ ; Nan, Lijun ¹ ; Liu, Junchao ¹ ; Yan, Haiyan ¹ ; Zhang, Dianpeng ² ; Han, Xinnian ³ ;

作者机构： 1.Shihezi Univ, Coll Food, Shihezi 832000, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, Inst Crops, Shihezi 832000, Peoples R China

关键词： Cabernet Sauvignon;Endophytes;Identification;Resveratrol;Screening

期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）

ISSN： 2193-1801

年卷期： 2016 年 5 卷

页码：

收录情况： SCI

摘要： Objectives: Obtain endophyte strains with effective resveratrol production from superior grapevine variety Cabernet Sauvignon in Xinjiang and determine related taxonomic position of the strain. Results: Seventy-three strains of endophytes, including 23 strains of bacteria, 14 ones of actinomycetes, 24 fungus and 12 yeasts, were isolated, respectively. The distribution law of endophytes was spring (30.14 %) = summer (30.14 %) < autumn (39.73 %) in different seasons, while the fruit (12.33 %) < leaf (20.55 %) < stem (32.88 %) < root (34.25 %) in different tissues and organs. From the 36 strains of endophytic fungi isolated, seven strains producing polyphenols were screened by ferric chloride-potassium ferricyanide color reaction. C2J6, stable genetic properties producing highly 1.48 mg L-1 of resveratrol, was identified as Aspergillus niger by 26S rDNA-ITS sequence analysis after thin-layer chromatography sieve analysis, ultra violet wavelength scanning and high performance liquid chromatography, respectively. Conclusions: There were the certain number and kinds of endophytes in the various tissues of Cabernet Sauvignon, which, to a certain extent, reflected the biological diversity of plant endophytes. The fact that the fungus C2J6 producing resveratrol in grape was acquired attested the special ability of the endophytes to produce the same or similar bioactive substances as the host plants.