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Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in Trametes gibbosa

文献类型： 外文期刊

作者： Chen, Yulan 1 ; Farooq, Akasha 1 ; Wei, Xieluyao 1 ; Qin, Leitao 1 ; Wang, Yong 1 ; Zhang, Lingzi 1 ; Xiang, Quanju 1 ; Zhao, Ke 1 ; Yu, Xiumei 1 ; Chen, Qiang 1 ; Gao, Xue 2 ; Nyima, Tashi 2 ; Penttinen, Petri 1 ; Gu, Yunfu 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Resources, Dept Microbiol, Chengdu, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Agr Resources & Environm Sci, Lhasa, Xizang, Peoples R China

关键词： phosphate solubilizing fungi; transcriptomic analysis; metabolomic analaysis; phosphorus solubilization mechanism; bio-phosphate fertilizer

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN：

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Introduction: Phosphorus (P) is a crucial growth-limiting nutrient in soil, much of which remains challenging for plants to absorb and use. Unlike chemical phosphate fertilizers, phosphate-solubilizing microorganisms (PSMs) offer a means to address available phosphorus deficiency without causing environmental harm. PSMs possess multiple mechanisms for phosphorus solubilization. Although the phosphorus-solubilizing mechanisms of phosphate-solubilizing bacteria (PSB) have been well characterized, the mechanisms utilized by phosphate-solubilizing fungi (PSF) remain largely unexplored. Methods: This study isolated a PSF strain, Trametes gibbosa T-41, from soil and evaluated its phosphorus solubilizing capacity with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources. The phosphorus solubilization, enzyme activity, and organic acid production of T-41 were measured. And the P-solubilizing mechanism conducted by transcriptomic and metabolomic analyses. Results and discussion: T-41 exhibited varying phosphorus solubilizing capacity when grown with organic (calcium phytin; Phytin-P) and inorganic (tricalcium phosphate; Ca-P) phosphorus sources (109.80 +/- 8.9 mg/L vs. 57.5 +/- 7.9 mg/L, p < 0.05). Compared with the Ca-P treatment, T-41 demonstrated a stronger alkaline phosphatase (ALP) production capacity under Phytin-P treatment (34.5 +/- 1.2 mu mol/L/h vs. 19.8 +/- 0.8 mu mol/L/h, p < 0.05). Meanwhile, the production of oxalic acid, maleic acid, and succinic acid was higher under Phytin-P treatment (p < 0.05). Transcriptomic and metabolomic analysis revealed that different phosphorus sources altered metabolic pathways such as galactose metabolism, glyoxylate and dicarboxylic acid metabolism, and ascorbate and aldolate metabolism. Key metabolites like myo-inositol, 2-oxoglutarate, and pyruvate were found to impact the performance of T. gibbosa T-41 differently under the two P sources. Notably, synthesis in Ca-P vs. Pytin-P, T-41 upregulated genes involved in myo-inositol synthesis, potentially enhancing its P-solubilizing ability. These results provide new insights into the molecular mechanisms of PSF at the transcriptomic and metabolomic levels, laying a theoretical foundation for the broader application of PSF as bio-phosphorus fertilizers in the future.

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