Development and Characterization of Novel Microsatellite Markers for the Peach Fruit Moth Carposina sasakii (Lepidoptera: Carposinidae) Using Next-Generation Sequencing
文献类型: 外文期刊
作者: Wang, You-Zhu 1 ; Cao, Li-Jun 1 ; Zhu, Jia-Ying 2 ; Wei, Shu-Jun 1 ;
作者机构: 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.Southwest Forestry Univ, Key Lab Forest Disaster Warning & Control Yunnan, Coll Forestry, Kunming 650224, Peoples R China
3.Beijing Forestry Univ, Coll Forestry, Beijing Key Lab Forest Pest Control, Beijing 100083, Peoples R China
关键词: simple sequence repeat;Carposina sasakii;peach fruit moth;next-generation sequencing
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2016 年 17 卷 3 期
页码:
收录情况: SCI
摘要: The peach fruit moth Carposina sasakii is an economically important pest on dozens of fruits from Rosaceae and Rhamnaceae in Northeast Asia. We developed novel microsatellite markers for C. sasakii from randomly sequenced regions of the genome using next-generation sequencing. In total, 95,153 microsatellite markers were isolated from 4.70 GB genomic sequences. Thirty-five polymorphic markers were developed by assessing in 63 individuals from two geographical populations. The allele numbers ranged from 2 to 9 with an average value of 4.60 per locus, while the polymorphism information content ranged from 0.075 to 0.696 with an average value of 0.407. Furthermore, the observed and expected heterozygosity varied from 0.000 to 0.677 and 0.062 to 0.771, respectively. The microsatellites developed provide abundant molecular markers for investigating genetic structure, genetic diversity, and existence of host-plant associated biotypes of C. sasakii.
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