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Improvement of Agrobacterium-mediated transformation efficiency of maize (Zea mays L.) genotype Hi-II by Optimizing Infection and Regeneration Conditions

文献类型： 外文期刊

作者： Xu, You 1 ; Ren, Wen 1 ; Liu, Ya 1 ; Zhao, Jiuran 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing 100097, Peoples R China

2.Taizhou Vocat Coll Sci & Technol, Taizhou 318000, Peoples R China

关键词： Maize (Zea mays L.);Hi-II;Agrobacterium tumefaciens;transformation

期刊名称：RESEARCH JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.233； 五年影响因子：0.252 ）

ISSN： 2278-4535

年卷期： 2016 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Improving Agrobacterium tumefaciens mediated transformation of maize would be beneficial for maize transgenic research. Although, maize genotype Hi-II is used in various maize transformation research efforts, improvements in the transformation frequency of this genotype are still needed. In the present study, the immature maize embryos were given heat-shock pretreatment prior to Agrobacterium-mediated transformation. About 9% increase in the transformation frequency was achieved when the embryos were subjected to 42 degrees C for 3 min or 38 degrees C for 9 min. It was also found that the use of 100 mg/L Casein Hydrolysate (CH) accelerated somatic embryogenesis in the initial period of regeneration. The combined use of 0.5 mg/L Naphthaleneacetic acid (NAA) and 2.0 mg/L Multi-effect triazole (MET) at different regenerate stage could effectively enhance root development and seedlings vigour to improve the survival rate of the transformed plantlets. Through integrating several methods can effectively improve the Hi-II genetic transformation efficiency.

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