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Immunity Elicited by an Experimental Vaccine Based on Recombinant Flagellin-Porcine Circovirus Type 2 Cap Fusion Protein in Piglets

文献类型： 外文期刊

作者： Zhu, Shanshan 1 ; Zhang, Chunyan 1 ; Wang, Jing 1 ; Wei, Li 1 ; Quan, Rong 1 ; Yang, Jiayu 2 ; Yan, Xu; Li, Zixuan; Sh 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Prevent & Control Infect Dis Live, Inst Anim Husb & Vet Med, 9 Shuguang Garden Middle Rd, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Prevent & Control Infect Dis Live, Inst Anim Husb & Vet Med, 9 Shuguang Garden Middle Rd, Beijing 100097, Peoples R

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2016 年 11 卷 2 期

页码：

收录情况： SCI

摘要： In a recent study, we reported that a recombinant protein from fusion expression of flagellin to porcine circovirus type 2 (PCV2) Cap induced robust humoral and cell-mediated immunity that afforded full protection for PCV2 infection using BALB/c mice. Here, we further evaluated the immunogenicity and protection of the recombinant protein using specific pathogen free (SPF) pigs. Twenty-five 3-week-old piglets without passively acquired immunity were divided into 5 groups. All piglets except negative controls were challenged with a virulent PCV2 at 21 days after booster vaccination and necropsied at 21 days post-challenge. Vaccination of piglets with the recombinant protein without adjuvant induced strong humoral and cellular immune responses as observed by high levels of PCV2-specific IgG antibodies and neutralizing antibodies, as well as frequencies of PCV2-specific IFN-gamma-secreting cells that conferred good protection against PCV2 challenge, with significant reduced PCV2 viremia, mild lesions, low PCV2 antigen-positive cells, as well as improved body weight gain, comparable to piglets vaccinated with a commercial PCV2 subunit vaccine. These results further demonstrated that the recombinant flagellin-Cap fusion protein is capable of inducing solid protective humoral and cellular immunity when administered to pigs, thereby becoming an effective PCV2 vaccine candidate for control of PCV2 infection.

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