The role of NADPH oxidase in the mud crab (Scylla paramamosain) in response to Vibrio parahaemolyticus infection
文献类型： 外文期刊
作者： Cheng, Chang-Hong 1 ; Dong, Jun-De 2 ; Fan, Si-Gang 1 ; Ma, Hong-Ling 1 ; Deng, Yi-Qin 1 ; Feng, Juan 1 ; Jiang, Jian-Jun 1 ; Guo, Zhi-Xun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Peoples R China
3.Sanya Trop Fisheries Res Inst, Sanya 572018, Peoples R China
关键词： Nox; ROS; Innate immunity; Antioxidant gene
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：4.3； 五年影响因子：4.1 ）
ISSN： 1532-0456
年卷期： 2025 年 292 卷
页码：
收录情况： SCI
摘要： Reactive oxygen species (ROS) are critical for maintaining cellular homeostasis and function. The main source of intracellular ROS depends on the NADPH oxidase (Nox) and plays crucial roles in immune deafens in animal. However, the function of Nox in crustaceans remains unclear. In the present study, a Nox gene from the mud crab (designated as Sp-Nox) was cloned and identified. The full length of Sp-Nox contained an open reading frame of 3939 bp encoding 1312 amino acids, a 5 ' untranslated region (UTR) of 420 bp, and a 3 ' UTR of 813 bp. The deduced amino acid sequences of Sp-Nox contained a typical flavin adenine dinucleotide (FAD) binding domain and a nicotinamide adenine dinucleotide (NAD) binding domain. Sp-Nox was widely expressed in all tested tissues, with the highest expression levels in the gill. Sp-Nox expression in hepatopancreas was significantly upregulated after V. parahaemolyticus infection. In order to know more about the regulation mechanism of Sp-Nox, RNA interference experiment was investigated. Knocking down Sp-Nox in vivo can significantly reduce the production of ROS and the expression levels of antioxidant-related genes (CAT and SOD). Moreover, Sp-Nox interference significantly increased the mortality of mud crabs after V. parahaemolyticus infection. All these results suggested that Sp-Nox played a crucial role in the defense against V. parahaemolyticus infection in crustaceans.
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