Genome-Wide Identification of the ClpB Gene Family in Tomato and Expression Analysis Under Heat Stress
文献类型： 外文期刊
作者： Zhang, Yuemei 1 ; Yang, Tailai 1 ; Han, Jiaxi 1 ; Su, Xiao 1 ; Cong, Yanqing 1 ; Zhou, Ming 2 ; Wang, Yan 1 ; Lin, Tao 1 ;
作者机构： 1.China Agr Univ, Coll Hort, Dept Vegetable Sci, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China
关键词：
Caseinolytic Protease B;
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 22 期
页码：
收录情况： SCI
摘要： Tomato is a widely grown horticultural crop, and its growth process is often affected by high temperatures. Caseinolytic Protease B (ClpB), a homologous protein to heat shock protein 101 (HSP101), plays a vital role in plant heat adaptation and development. In this study, we identified six SlClpB genes in tomatoes, distributed across four chromosomes. Collinearity analysis revealed that the gene pairs SlClpB-2 and SlClpB-3A, as well as SlClpB-3C and SlClpB-12, resulted from segmental duplication events. Phylogenetic and motif analyses showed that ClpB proteins possess highly conserved domains across different species. We used RNA-seq data to analyze the expression patterns of the ClpB family. Among them, SlClpB-3A and SlClpB-12 exhibited increased expression in multiple tissues under heat stress. Specifically, SlClpB-2, SlClpB-3A, and SlClpB-3C were highly expressed in the fruit orange stage and in flower buds under heat treatment, while in seedlings, SlClpB-2 and SlClpB-3A exhibited heat-induced expression. Real-time quantitative fluorescent PCR (qRT-PCR) results showed that the expression of SlClpB-2 and SlClpB-3A was significantly increased under heat stress in the leaves and buds of Ailsa Craig, Micro-Tom, and M82. Overall, our findings provide valuable insights into the regulatory mechanisms of SlClpB genes in response to heat stress.
- 相关文献
作者其他论文 更多>>
-
Insights into Phylogeny, Taxonomy, Origins and Evolution of Crataegus and Mespilus, Based on Comparative Chloroplast Genome Analysis
作者：Meng, Jiaxin;Wang, Yan;Dong, Ningguang;Song, Han;Dong, Wenxuan
关键词：
Crataegus ; chloroplast genome; phylogeny; molecular dating; biogeography inference -
Advancing grape breeding through an in vitro embryo germination technique without cold stratification
作者：Liu, Zhenhua;Wang, Yan;Wang, Huiling;Sun, Lei;Yan, Ailing;Ren, Jiancheng;Wang, Xiaoyue;Xu, Haiying;Song, Jing;Guan, Pingyin
关键词：Grape; Seed dormancy; Germination; Embryo culture
-
A natural variation of flavone synthase II gene enhances flavone accumulation and confers drought adaptation in chrysanthemum
作者：Luo, Jiayi;Han, Mingzheng;Song, Zhenzhen;Zhang, Haixia;Zhao, Yafei;Ma, Chao;Luo, Chang;Huang, Conglin;Wang, Qinrui;Gao, Qiang;Lin, Tao
关键词：Chrysanthemum; drought; flavone; FNSII; GWAS
-
Molecular breeding of tomato: Advances and challenges
作者：Du, Minmin;Du, Minmin;Sun, Chuanlong;Deng, Lei;Li, Chang-Bao;Li, Chuanyou;Du, Minmin;Sun, Chuanlong;Li, Chuanyou;Deng, Lei;Li, Chang-Bao;Li, Chuanyou;Zhou, Ming;Li, Chang-Bao;Li, Junming;Du, Yongchen;Ye, Zhibiao;Huang, Sanwen;Huang, Sanwen;Li, Tianlai;Yu, Jingquan
关键词：genome editing; genome sequencing; germplasm; molecular breeding; tomato
-
Emerging Pollutants
作者：Wang, Yawei;Zhang, Qiurui;Shi, Yali;Shi, Jianbo;Qu, Guangbo;Qiao, Min;Liu, Mei;Liu, Guorui;Liu, Yanna;Yang, Lili;Yang, Xiaoxi;Yang, Ruiqiang;Zheng, Minghui;Jiang, Lu;Yao, Linlin;He, Yong;Cai, Yaqi;Wei, Dongbin;Liao, Chunyang;Jiang, Guibin;Yu, Nanyang;Wei, Si;Ji, Rong;Pan, Bingcai;Liu, Jianguo;Qiu, Xinghua;Lu, Rongjing;Chen, Jiazhe;Cheng, Zhen;Zhu, Chuhong;Jiang, Longfei;Mai, Bixian;Zhang, Gan;Luo, Xiaojun;Luo, Chunling;Jin, Biao;Zhao, Shizhen;Zeng, Yanhong;Zhu, Yumin;Yang, Rongyan;Zhang, Ying;Chen, Xin;Zhu, Lingyan;Hua, Jianghuan;Yang, Lihua;Zhou, Bingsheng;Guo, Yongyong;Liang, Chengqian;Han, Jian;Wang, Yan;Zhang, Quan;Zhao, Meirong;Mo, Xunjie;Zhu, Ying;Sheng, Nan;Dai, Jiayin;Ying, Guangguo;Zhang, Qianqian;Gao, Chuanzi;Qiu, Wenhui;Tang, Shuqin;Chen, Da;Chen, Jingwen;Cui, Yunhan;Tian, Sinuo;Li, Cheng;Fang, Mingliang;Wang, Yuan;Liu, Nannan;Yang, Pan;Chen, Hexia;Zhang, Zhen;Liu, Guorui;Yang, Ruiqiang;Lin, Bingcheng;Zheng, Minghui;Jiang, Guibin;Zhao, Fanrong;Yao, Jingzhi;Tan, Hongli;Liu, Guorui;Yang, Lili;Yang, Ruiqiang;Zheng, Minghui;Jiang, Guibin;Yang, Lihua;Zhou, Bingsheng;Guo, Yongyong;Han, Jian;Hua, Jianghuan;Liang, Chengqian;Zhao, Jincai;Shi, Jianbo
关键词：emerging pollutants; occurrence level; environmental behavior; ecological risks; control strategies
-
Interaction of VvDELLA2 and VvCEB1 Mediates Expression of Expansion-Related Gene during GA-Induced Enlargement of Grape Fruit
作者：Liu, Zhenhua;Wang, Yan;Sun, Lei;Liu, Zhenhua;Wang, Yan;Sun, Lei;Guan, Pingyin;Hu, Jianfang
关键词：GA(3); cell expansion; VvDELLA2; VvCEB1; grape; early fruit enlargement
-
Integrative Analysis of Metabolome and Transcriptome Reveals Molecular Insight into Metabolomic Variations during Hawthorn Fruit Development
作者：Wang, Yan;Hao, Ruixin;Guo, Rongkun;Nong, Huilan;Qin, Yu;Dong, Ningguang
关键词：broadly targeted metabolome; metabolites; fruit development and ripening; flavonoids; hawthorn; transcriptome
