文献类型： 外文期刊

作者： Lv, Xue-Ze ¹ ; Wang, Hong-Jun ¹ ; Chen, Xiao-Ling ¹ ; Gong, Yu-Mei ¹ ; Liang, Yu-Rong ¹ ; He, Yun-Xia ² ; Zhang, Pei-J ¹ ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Live, 9 Shuguang Garden Middle Rd, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Live, 9 Shuguang Garden Middle Rd, Beijing 100097, Peoples R

关键词： Apg;aroA;gene identification;protein structure;molecule evolution

期刊名称：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE （ 影响因子：0.166； 五年影响因子：0.621 ）

ISSN： 1940-5901

年卷期： 2016 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Objectives: A convenient and efficient method for the clone of unknown full-length gene was established and the structure and molecule evolution of full-length aroA gene of Avibacterium paragallinarum were evaluated. Methods: This experiment took an international standard strain 145 (C-3) of Apg as template, designed degenerate primers according to aroA gene with CODEHOP software. The improved chromosome walking was used to amplify the whole gene sequence of aroA. Some structure researches on the nucleicacid and coding protein sequence have been done. Besides, we also did some research on molecular evolution analysis with other serotypes and related bacteria by sequence alignment. Results: Complete aroA gene of Avibacterium paragallinarum was obtained, which is 1 293 bp. The length of the gene encoded product is 430aa, which have three transmembrane domains and five potential epitopes. The homology of amino acid sequences is 91.4%-100% between different serotypes experiment strains, and the nucleic acid homology is over 74.2% between bacteria of the same family. Conclusions: This trial has combined the degenerate PCR and improved chromosome walking for the research of avibacterium coli aroA genes complete sequence for the first time, and obtained the expected results. It laid the foundation for the study of biological activity of its encoded protein and building avibacterium bacilli aroA deletion attenuated strains.