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PROTEIN SECONDARY STRUCTURE CHANGES OF WATERMELON JUICE TREATED WITH HIGH HYDROSTATIC PRESSURE BY FTIR SPECTROSCOPY

文献类型： 外文期刊

作者： Liu, Qing 1 ; Wang, Ru-Fu 1 ; Zhang, Bo-Bo 1 ; Zhao, Xiao-Yan 3 ; Wang, Dan 3 ; Zhang, Chao 3 ;

作者机构： 1.Shanxi Agr Univ, Coll Food Sci & Engn, Taigu 030801, Shanxi, Peoples R China

2.Beijing Vocat Coll Agr, Dept Biol & Food Technol, Beijing 102442, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

4.Natl Engn Res Vegetable, Beijing 100097, Peoples R China

期刊名称：JOURNAL OF FOOD PROCESS ENGINEERING （ 影响因子：2.356； 五年影响因子：2.417 ）

ISSN： 0145-8876

年卷期： 2014 年 37 卷 6 期

页码：

收录情况： SCI

摘要： Watermelon (Citrullus lanatus) is rich in nutrients, such as lycopene, ascorbic acid and amino acids. It also has effect on thirst quenching, diuretic action and lowering the blood pressure. Traditional thermal sterilization will destroy the active ingredient of watermelon juice and lead to an odd smell, which seriously affects the sensory juice quality and the acceptability of consumers. High hydrostatic pressure (HHP), a nonthermal processing technology, can realize food sterilization process at room temperature or lower temperature. This study aims to study the HHP effect on protein secondary structure of watermelon juice. Crude protein of the treatment group was extracted after the HPP device treatment. Different degree, time, temperature and interval frequency of HHP were imposed on the watermelon juice protein, followed by characterization of the protein secondary structure of watermelon juice by the Fourier transform infrared spectroscopy. The absorption of protein in watermelon juice decreased with the pressure of higher degree, longer time, higher temperature and more interval frequency. The -helix content became lower after treatment; and -sheet level was decreased while random coil increased after 400MPa treatments for 20, 40 and 60min compared with control. The trends of -turn values changed variously in different treatment groups. HHP treatment can change the secondary structures of proteins and affect the content of -helix with higher degree of HHP, longer time, higher temperature and more interval frequency. Practical ApplicationsThis study pays attention on the proper sterilization for watermelon juice products. HHP treatment on watermelon juice at a condition of 400MPa, 30C for twice interval of 10min may be the best choice. Under this condition, HHP treatment performs better inactivation effects on endogenous enzymes and lead to relatively less adverse effects on flavor of products.

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