您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Adventitious shoot regeneration from leaves of apple rootstock 'Pingyitiancha' (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers

文献类型： 外文期刊

作者： Jin, Wanmei 1 ; Wang, Yuanhua 1 ; Wang, Hua 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

关键词： Plant growth regulators;dark;rooting;explant orientation;molecular marker

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2014 年 94 卷 8 期

页码：

收录情况： SCI

摘要： Apple is one of the major fruit tree species in China, its cultivation area and total output rank first in the world. 'Pingyitiancha' (Malus hupehensis var. pinyiensis) is a widely used rootstock for apple cultivation in China. Several factors affecting leaf regeneration were investigated. In this study, a successful adventitious shoot regeneration protocol for this cultivar was established. 'Pingyitiancha' leaves were a suitable source of explants for regeneration of adventitious shoots. The optimal adventitious shoot regeneration protocol involved subculturing seedling leaves for 30-35 d. The optimum medium was Murashige and Skoog (MS) medium containing 2.0 mg L-1 thidiazuron and 0.2 mg L-1 indole-3-butyric acid. Explants with the abaxial surface in contact with the medium kept for 14 d in the dark showed the highest regeneration percentage of adventitious shoots of explants (100%), and produced an average of 3.6 shoots per regenerating explant. Shoots regenerated from leaves were rooted on half-strength MS medium containing 0.4 mg L-1 1-naphthalene acetic acid. The rooting percentage was 94.4%. Using SSR markers, all banding profiles from regenerated plantlets were monomorphic and same to those of the mother plant. It showed that the uniformity of the in vitro regenerated plantlets was maintained.

  • 相关文献

[1]Establishment and Application of Ty-2 Molecular Marker in Tomatoes. Ruixing Yatng,Shuwen lv,Min Chai,Haitao Li. 2012

[2]Histological and endogenous plant growth regulators changes associated with adventitious shoot regeneration from in vitro leaf explants of strawberry (Fragaria x ananassa cv. 'Honeoye'). Wang, Hua,Li, Maofu,Yang, Yuan,Dong, Jing,Jin, Wanmei.

[3]Screening for the molecular marker linked to saucer gene of peach fruit shape. Guo, J,Jiang, Q,Zhang, K,Zhao, J,Yang, Y. 2002

[4]Establishment and Application of Ty-2 Molecular Marker in Tomatoes. Yang, Ruixing,Li, Haitao,Lv, Shuwen,Li, Haitao,Chai, Min. 2012

[5]Molecular markers for tracking variation in lipoxygenase activity in wheat breeding. Geng, Hongwei,Zhang, Yan,He, Zhonghu,Xia, Xianchun,Geng, Hongwei,Qu, Yanying,He, Zhonghu,Zhang, Liping,Appels, Rudi.

[6]Examining genetic relationships of Chinese Pleurotus ostreatus cultivars by combined RAPD and SRAP markers. Yin, Yonggang,Liu, Yu,Wang, Shouxian,Zhao, Shuang,Xu, Feng,Yin, Yonggang. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台