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Efficient transformation and expression of the glucanase gene from Bacillus megaterium in the biocontrol strain Streptomyces lydicus A02

文献类型： 外文期刊

作者： Wu, Huiling 1 ; Dong, Dan 1 ; Li, Jinjin 1 ; Liu, Weicheng 1 ; Liu, Ting 1 ; Zhang, Taotao 1 ; Tian, Zhaofeng 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： glucanase;antifungal activity;Streptomyces lydicus;natamycin production

期刊名称：BIOCONTROL SCIENCE AND TECHNOLOGY （ 影响因子：1.665； 五年影响因子：1.616 ）

ISSN： 0958-3157

年卷期： 2014 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Streptomyces have been used extensively as the biocontrol agents due to their ability to produce various antimicrobial compounds, such as antibiotics and hydrolytic enzymes. Streptomyces lydicus strain A02, which was isolated from the soil of suburban forest field in Beijing (China), is capable of producing natamycin and has proved to be a potential biocontrol agent to several plant fungal diseases, including wilts caused by Fusarium oxysporum f. spp. However, hydrolytic enzymes like glucanase have not been detected in S. lydicus A02 on CMC-Na plates by congo red staining. Glucanase, a pathogenesis-related (PR) protein, degrades fungal cell walls and has been widely used as antifungal agent in plant protection. Therefore, a recombinant S. lydicus expressing a glucanase gene, which was cloned from the biocontrol strain Bacillus megaterium L103 and driven by the Streptomyces erythraea ermE* promoter, was constructed in this study. The engineered S. lydicus AG02 shared a similar yield of natamycin with the wild-type A02 strain. Compared to the wild-type strain A02, the engineered S. lydicus AG02 had a remarkably higher glucanase activity, as well as antifungal activity to F. oxysporum f. sp. conglutinans, F. oxysporum f. sp. niveum and Rhizoctonia cerealis. This demonstrated the improved biocontrol effect of S. lydicus AG02 attributed to transforming the exogenous glucanase from B. megaterium, which acted synergistically with natamycin to increase the antifungal activity of the strain.

  • 相关文献

[1]Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites. Wu, Huiling,Li, Jinjin,Dong, Dan,Liu, Ting,Zhang, Taotao,Zhang, Dianpeng,Liu, Weicheng.

[2]Expression of Paenibacillus polymyxa beta-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea. Li, Jinjin,Liu, Weicheng,Dong, Dan,Liu, Ting,Zhang, Taotao,Lu, Caige,Liu, Dewen,Zhang, Dianpeng,Wu, Huiling,Luo, Lijin.

[3]Construction of Streptomyces lydicus A01 transformant with the chit33 gene from Trichoderma harzianum CECT2413 and its biocontrol effect on Fusaria. Wu Qiong,Li YingYing,Li YaQian,Lin ZhenYa,Wang Meng,Chen Jie,Bai LinQuan,Liu WeiCheng,Lu CaiGe,Xue ChunSheng. 2013

[4]SlnM gene overexpression with different promoters on natamycin production in Streptomyces lydicus A02. Wu, Huiling,Liu, Weicheng,Dong, Dan,Li, Jinjin,Zhang, Dianpeng,Lu, Caige. 2014

[5]IDENTIFICATION OF AN ANTIFUNGAL METABOLITE PRODUCED BY A POTENTIAL BIOCONTROL ACTINOMYCES STRAIN A01. Lu, Cai Ge,Liu, Wei Cheng,Qiu, Ji Yan,Liu, Ting,Liu, De Wen,Lu, Cai Ge,Wang, Hui Min. 2008

[6]Construction of a Streptomyces lydicus A01 transformant with a chit42 gene from Trichoderma harzianum P1 and evaluation of its biocontrol activity against Botrytis cinerea. Wu, Qiong,Li, Yingying,Li, Yaqian,Fu, Kehe,Yu, Chuanjin,Chen, Jie,Bai, Linquan,Liu, Weicheng,Lu, Caige.

[7]Optimization of the Fermentation Process for Streptomyces lydicus A02 Producing Natamycin. Weicheng Liu,Ting Liu,Caige Lu,Taotao Zhang,Dewen Liu,Yupeng Hua. 2012

[8]Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot. Wang Qing,Zuo Jin-hua,Wang Qian,Na Yang,Gao Li-pu. 2015

[9]Characterization of Phenolic Compounds from Early and Late Ripening Sweet Cherries and Their Antioxidant and Antifungal Activities. Wang, Meng,Jiang, Nan,Wang, Yao,Jiang, Dongmei,Feng, Xiaoyuan,Wang, Meng,Jiang, Nan,Wang, Yao,Jiang, Dongmei,Feng, Xiaoyuan.

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