Specific TonB-ExbB-ExbD energy transduction systems required for ferric enterobactin acquisition in Campylobacter
文献类型： 外文期刊
作者： Zeng, Ximin 1 ; Xu, Fuzhou 1 ; Lin, Jun 1 ;
作者机构： 1.Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA
2.Beijing Acad Agr & Forestry Sci, Inst Anim Sci & Vet Med, Beijing, Peoples R China
关键词： iron uptake;molecular mechanism;siderophore
期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）
ISSN： 0378-1097
年卷期： 2013 年 347 卷 1 期
页码：
收录情况： SCI
摘要： Ferric enterobactin (FeEnt) acquisition plays a critical role in the pathophysiology of Campylobacter, the leading bacterial cause of human gastroenteritis in industrialized countries. In Campylobacter, the surface-exposed receptor, CfrA or CfrB, functions as a gatekeeper' for initial binding of FeEnt. Subsequent transport across the outer membrane is energized by TonB-ExbB-ExbD energy transduction systems. Although there are up to three TonB-ExbB-ExbD systems in Campylobacter, the cognate components of TonB-ExbB-ExbD for FeEnt acquisition are still largely unknown. In this study, we addressed this issue using complementary molecular approaches: comparative genomic analysis, random transposon mutagenesis and site-directed mutagenesis in two representative C.jejuni strains, NCTC 11168 and 81-176. We demonstrated that CfrB could interact with either TonB2 or TonB3 for efficient Ent-mediated iron acquisition. However, TonB3 is a dominant player in the CfrA-dependent pathway. The ExbB2 and ExbD2 components were essential for both CfrA- and CfrB-dependent FeEnt acquisition. Sequences analysis identified potential TonB boxes in CfrA and CfrB, and the corresponding binding sites in TonB. In conclusion, these findings identify specific TonB-ExbB-ExbD energy transduction components required for FeEnt acquisition, and provide insights into the complex molecular interactions of FeEnt acquisition systems in Campylobacter.
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