Examining genetic relationships of Chinese Pleurotus ostreatus cultivars by combined RAPD and SRAP markers
文献类型: 外文期刊
作者: Yin, Yonggang 1 ; Liu, Yu 1 ; Wang, Shouxian 1 ; Zhao, Shuang 1 ; Xu, Feng 1 ;
作者机构: 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.Agr Univ Hebei, Coll Hort, Baoding 071001, Hebei, Peoples R China
关键词: Genetic diversity;Molecular marker;Polymorphism;UPGMA
期刊名称:MYCOSCIENCE ( 影响因子:1.223; 五年影响因子:1.266 )
ISSN: 1340-3540
年卷期: 2013 年 54 卷 3 期
页码:
收录情况: SCI
摘要: Combined randomly amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) were used to assess the genetic diversity of Pleurotus ostreatus strains cultivated in China. For the RAPD and SPAP analyses, 479 and 282 polymorphic bands were obtained from 20 P. ostreatus strains using 20 and 13 selected primers or primer pairs, respectively. A combined RAPD/SRAP dendrogram grouped the 20 strains into five clades with a coefficient of 0.690. The comparison of RAPD and SRAP was evaluated in the present study. The combined RAPD/SRAP markers provided reliable information regarding the relationships among the P. ostreatus strains. (C) 2012 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.
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