Inheritance of downy mildew resistance at different developmental stages in Chinese cabbage via the leaf disk test
文献类型： 外文期刊
作者： Zhang, Shengju 1 ; Yu, Shuancang 1 ; Zhang, Fenglan 1 ; Si, Longting 2 ; Yu, Yangjun 1 ; Zhao, Xiuyun 1 ; Zhang, Deshu 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Shenyang Agr Univ, Dept Hort, Shenyang 110161, Peoples R China
关键词： Brassica rapa L. ssp pekinensis;disease resistance identification;double haploid population;Hyaloperonospora parasitica;in vitro test
期刊名称：HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY （ 影响因子：1.842； 五年影响因子：2.083 ）
ISSN： 2211-3452
年卷期： 2012 年 53 卷 5 期
页码：
收录情况： SCI
摘要： Downy mildew, caused by Hyaloperonospora parasitica, is a destructive disease of the Chinese cabbage (Brassica rapa ssp. pekinensis). A rapid resistance/susceptibility test for H. parasitica was established by the inoculation of a leaf disk test. Four conditions were tested and the optimal condition was found when the inoculated leaf disk was placed into dark conditions at 16 degrees C for the first 24 hours after inoculation, followed by 5 days of light at 20 degrees C and 23 degrees C for 4 hours and 5 hours, respectively, dark at 16 degrees C and 12 degrees C for 3 hours and 12 hours, respectively, and a final temperature of 16 degrees C for 24 hours. There was a good correlation between the resistance levels of leaf disks and the resistance of seedlings or adult plants, which indicated that testing leaf disks should be the preferred methods to predict the resistance of adult plants. Using this method, downy mildew resistance was investigated in a double haploid (DH) population at four developmental stages. The results showed that the genetic pattern, which was deduced from the DH segregation, was relatively similar, but varied slightly during plant development.
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