北京市农林科学院机构知识库

Establishment of AFLP Analysis System in Spinach (Spinacia oleracea L.)

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文献类型：外文期刊

作者： Meng, Shuchun ¹ ; Liu, Ling ¹ ; Mei, Yi ² ; Guo, Jun ² ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

2.Yancheng Acad Agr Sci, Yancheng 224002, Peoples R China

关键词： spinach (Spinacia oleracea L.);AFLP;influencing factors

期刊名称：INTERNATIONAL SYMPOSIUM ON VEGETABLE PRODUCTION, QUALITY AND PROCESS STANDARDIZATION IN CHAIN: A WORLDWIDE PERSPECTIVE

ISSN： 0567-7572

年卷期： 2012 年 944 卷

页码：

收录情况： SCI

摘要： In this study total genetic DNA from tender leaves of spinach (Spinacia oleracea) was extracted with improved CTAB. An AFLP (amplified fragment length polymorphism) analysis system was established by optimizing the concentration of template DNA, DNA restriction reaction, ligase reaction, pre-amplification and selective amplification. The results were obtained as follow: (1) The effect of restriction enzyme was better in PCR instrument than in water-bath, the reaction time and the concentration of DNA were not sensitive to restriction enzyme. (2) The best reaction system in 20 mu l total volume contained Mg2+ (25 mmol/L) 1.2 mu l, dNTPs (2.5 mmol/L) 1.6 mu l and Taq-polymerase (5U/mu l) 0.2 mu l. This paper provided a method for further study in the field of genetic relative analysis of spinach (Spinacia oleracea L.) cultivars and genetic breeding.

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