Detection of herbicide-resistant maize by using loop-mediated isothermal amplification of the pat selectable marker gene
文献类型： 外文期刊
作者： Chen, Jinsong 1 ; Huang, Conglin 1 ; Zhang, Xiuhai 1 ; Yu, Rong 2 ; Wu, Zhongyi 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
2.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
关键词： Herbicide-resistant maize;loop-mediated isothermal amplification (LAMP);transgenic maize detection;pat gene
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 75 期
页码：
收录情况： SCI
摘要： Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that reagents react to under isothermal conditions with high specificity, efficiency and rapidity. We used LAMP for detection of herbicide-resistant maize, which is a widespread genetically modified crop. Pat gene, a widely used selectable marker, was amplified by a set of four special primers that recognized six distinct sequences of the target. We studied the optimized conditions for LAMP using different Mg(2+), dNTPs, betaine and primers concentrations. Furthermore, we tested the sensitivity and specificity of LAMP as compared to PCR. This work shows that the LAMP method can detect a specific pat gene from herbicide-resistant maize and their test results are consistent with the results of the conventional PCR methods. However, LAMP assay results were found to be more sensitive than the conventional PCR.
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