文献类型: 外文期刊
作者: Li, Shuang 1 ; Ren, Xia 2 ; Huang, Conglin 2 ; Wu, Zhongyi 2 ; Jia, Guixia 1 ; Zhang, Xiuhai 2 ;
作者机构: 1.Beijing Forestry Univ, Coll Landscape Architecture, Natl Engn Res Ctr Floriculture, Beijing, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing, Peoples R China
关键词: FT;viral vector;lily
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 42 期
页码:
收录情况: SCI
摘要: FT is thought to be the florigen in plants. In this research, a new method for promoting lily flowering was introduced. The function of FT gene cloned from Arabidopsis on promoting lily flowering was analyzed. pET-30a-FT vector was constructed to indicate the expression of FT:eGFP fuse protein in prokaryotic cells. FT: eGFP was also constructed into plant virus vector-pGR106. Agrobacterium GV3101 harboring pGR106-FT was injected into lily. The injected lily showed early flowering when compared with the control plants. The detection of eGFP and PCR analysis indicated that the virus harboring FT: eGFP was replicated and expressed in the host plants. The results showed that FT: eGFP fuse protein functioned in promoting lily flowering.
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