文献类型： 外文期刊

作者： Yang Yan-Mei ¹ ; Li Xia ⁴ ; Yang Hui ⁴ ; Qian Yuan ⁵ ; Zhang You ⁵ ; Huo Yan ¹ ; Fang Rong-Xiang ¹ ; Chen Xiao-Ying ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China

3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

5.Capital Inst Pediat, Virol Lab, Beijing 100020, Peoples R China

关键词： rotavirus;bioengineering vaccine;multi-epitopes

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 2011 年 38 卷 1 期

页码：

收录情况： SCI

摘要： The full length cDNAs of rotavirus structural protein genes, vp4, vp6 and vp7 were cloned from the rotavirus infected child stool specimen in Beijing through RT-PCR. The protein sequences and their antigenic determinants were predicted. According to the epitope peptide sequences, 4 epitopes from these structural proteins were chosen, a DNA fragment encoding all these epitopes was synthesized and cloned into the prokaryotic expression vector. Multiple epitope protein (rotavirus multiple epitopes, RME) expressed in E. coli can be recognized by the polyclonal antibody of rotavirus, and induce immune response in mice. The specific antibody IgG induced by RME can recognize human rotavirus (Wa strain), RME itself as well as individual epitope peptides. The antibody titer of IgG to RME is high (1: 40 000), while the titers to EV4, EV6 or EV7 are in a range of 1 : 10 000 to 1 : 20 000. However the IgG titer to the Wa strain is lower, i.e., 1 : 2 500. Intriguingly, the RME-induced IgG can neutralize the Wa strain rotavirus challenge in the MAC 145 cell line. This research has laid the foundations of producing effective bioengineering vaccines to rotavirus.