文献类型： 外文期刊

作者： Yang YanMei ¹ ; Li Xia ⁴ ; Yang Hui ⁴ ; Qian Yuan ⁵ ; Zhang You ⁵ ; Fang RongXiang ¹ ; Chen XiaoYing ¹ ;

作者机构： 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Microbiol, Beijing 100101, Peoples R China

2.Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China

3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

5.Capital Inst Pediat, Virol Lab, Beijing 100020, Peoples R China

关键词： rotavirus;virus-like particles;transgenic plant;oral vaccine;immunogenicity

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2011 年 54 卷 1 期

页码：

收录情况： SCI

摘要： The human pathogen, group A rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. There is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. Plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. Virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live attenuated viral vaccines. In this study, the rotavirus capsid proteins VP2, VP6 and VP7 were co-expressed in transgenic tobacco plants, and their expression levels, formation of rotavirus-like particles (RV VLPs) and immunogenicity were extensively studied. Quantitative real-time RT-PCR and Western blot analysis revealed that the expression level of vp6 was the highest while vp7 was expressed at the lowest levels. The RV VLPs were purified from transgenic tobacco plants and analyzed by electron microscopy and Western blot. Results indicated that the plant-derived VP2, VP6 and VP7 proteins self-assembled into 2/6 or 2/6/7 RV VLPs with a diameter of 60-80 nm. When orally delivered into mice with cholera toxin as an adjuvant, the total soluble protein extracted from transgenic tobacco plants induced rotavirus-specific antibodies comparable with those of attenuated rotavirus vaccines, while VP 2/6/7 induced higher serum IgG and fecal IgA titers compared with VP 2/6.