文献类型: 外文期刊
作者: Zhang Chao 1 ; Ma Yue 1 ; Zhao Xiao-Yan 1 ; Zhang Hui 2 ; Yao Hui-Yuan 2 ;
作者机构: 1.Beijing Acad Agr & Forestry Sci, Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
关键词: Winter wheat;bran;antifreeze protein;molecular weight;primary structure
期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )
ISSN: 0253-3820
年卷期: 2009 年 37 卷 5 期
页码:
收录情况: SCI
摘要: The primary structure of winter-wheat-bran antifreeze protein(TaAFP) is the foundation of its higher structure and functionality. Hence, the primary structure of TaAFP was determined by the combination of N-terminal sequencing and mass fingerprint overlapping analysis. The molecular weight of TaAFP was 13637.711 Da determined by MALDI-TOF-MS analysis, similar to that from SDS-PAGE analysis. TaAFP was cleaved by trypsin, hydroxylamine, and chymotrypsin, respectively. The cleaved peptides were further analyzed and connected to acquire its primary structure as MARKVIALAFLLLLTISLSKSNAARVKYNGGESGGGG GGGGGGGGGGNGSGSGSGYGYNYGKGGGQSGGGQGGGGGGGGGGGSNGSGSGSGYGYGYGQGNGGAQGQ GSGGGGGGGGGGGGGGSGQGSGSGYGYGYGKGGGGGGGGGGDGGGGGGGGSAYVGRHE, with overlap rate of 100%. Homogeneity analysis showed TaAFP was related with the cellular wall and cold resistance.
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