A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens
文献类型： 外文期刊
作者： Sun, H. 1 ; Miao, D. 2 ; Zhang, P. 2 ; Gong, Y. 2 ; Blackall, P. J. 2 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husbandry & Vet Sci, Beijing 100089, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Anim Husbandry & Vet Sci, Beijing 100089, Peoples R China; Dept Primary Ind & Fisheries, Anim Res Inst, Yeerongpilly, Qld 4105, Australia
关键词： blocking ELISA;HI;avibacterium paragallinarum
期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）
ISSN： 1045-1056
年卷期： 2007 年 35 卷 4 期
页码：
收录情况： SCI
摘要： The ability of blocking ELISAs and haemagglutination-inhibition (HI) tests to detect antibodies in sera from chickens challenged with either Avibacterium (Haemophilus) paragallinarum isolate Hp8 (serovar A) or H668 (serovar C) was compared. Serum samples were examined weekly over the 9 weeks following infection. The results showed that the positive rate of serovar A specific antibody in the B-ELISA remained at 100% from the second week to the ninth week. In chickens given the serovar C challenge, the highest positive rate of serovar C specific antibody in the B-ELISA appeared at the seventh week (60% positive) and was then followed by a rapid decrease. The B-ELISA gave significantly more positives at weeks 2, 3, 7, 8 and 9 post-infection for serovar A and at week 7 post-infection for serovar C. In qualitative terms, for both serovar A and serovar C infections, the HI tests gave a lower percentage of positive sera at all time points except at 9 weeks post-infection with serovar C. The highest positive rate for serovar A HI antibodies was 70% of sera at the fourth and fifth weeks post-infection. The highest rate of serovar C HI antibodies was 20% at the fifth and sixth weeks post-infection. The results have provided further evidence of the suitability of the serovar A and C B-ELISAs for the diagnosis of infectious coryza. (C) 2007 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.
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