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Molecular epidemiology of two fowl cholera outbreaks on a free-range chicken layer farm

文献类型: 外文期刊

作者: Zhang, P 1 ; Fegan, N 2 ; Fraser, I 2 ; Duffy, P 2 ; Bowles, RE 2 ; Gordon, A 2 ; Ketterer, PJ 2 ; Shinwari, W 2 ; Blackall, 1 ;

作者机构: 1.Queensland Dept Primary Ind, Anim Res Inst, Moorooka, Qld 4105, Australia

2.Queensland Dept Primary Ind, Anim Res Inst, Moorooka, Qld 4105, Australia; Queensland Dept Primary Ind, Agcy Food & Fibre Sci, Yeerongpilly, Qld 4105, Australia; Queensland Dept Primary Ind, Anim & Plant Hlth Serv, Yeerongpilly, Qld 4105, Australia; Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Sci, Beijing, Peoples R China; Queensland Dept Primary Ind, Anim Res Inst, Yeerongpilly, Qld 4105, Australia

期刊名称:JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION ( 影响因子:1.279; 五年影响因子:1.522 )

ISSN: 1040-6387

年卷期: 2004 年 16 卷 5 期

页码:

收录情况: SCI

摘要: Two outbreaks of fowl cholera on a multiage free-range egg farm were investigated. The outbreaks occurred in 1994 and 2002. A total of 22 strains of Pasteurella multocida were available for study, 11 from the 1994 outbreak and 11 from the 2002 outbreak. Lesions typical of acute fowl cholera were seen in the 1994 outbreak, whereas both acute and chronic fowl cholera occurred in the 2002 outbreak. The isolates were examined in an extended phenotypic typing methodology, by a P. multocida-specific polymerase chain reaction (PCR), by the Heddleston somatic serotyping scheme, and by restriction endonuclease analysis (REA) typing using the enzyme HpaII. All 22 strains had the same phenotypic properties, all were confirmed as P. multocida by PCR, all were Heddleston serovar 4, and all had the same REA pattern. The results indicate that these 2 outbreaks were caused by the same clone of P. multocida-despite the 8-year time period between the outbreaks.

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