文献类型： 外文期刊

作者： Zhang, P ¹ ; Blackall, PJ ² ; Yamaguchi, T ³ ; Iritani, Y ⁴ ;

作者机构： 1.Queensland Poultry Res & Dev Ctr, Anim Res Inst, Moorooka, Qld 4105, Australia

2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Sci, Beijing, Peoples R China

3.Shionogi & Co Ltd, Chuo Ku, Osaka 541, Japan

4.Shionogi & Co Ltd, Fukushima Ku, Osaka 553, Japan

关键词： ELISA;Haemophilus paragallinarum;serology;infectious coryza

期刊名称：AVIAN DISEASES （ 影响因子：1.577； 五年影响因子：1.915 ）

ISSN： 0005-2086

年卷期： 1999 年 43 卷 1 期

页码：

收录情况： SCI

摘要： Two monoclonal antibody-blocking enzyme-linked immunosorbent assays (B-ELISAs) were developed to detect serovar-specific antibodies to Haemophilus paragallinarum. One assay detected antibodies against serovar A and the other antibodies against serovar C. The assays were evaluated with sera derived from disease-free chickens as well as chickens experimentally immunized and/or challenged with H. paragallinarum strains 0083 (serovar A), Modesto (serovar C), or HP31 (serovar C). When rested with 440 negative sera (170 from a specific-pathogen-free and 30 from each of nine commercial layer flocks), both tests gave only a single false-positive reaction. The use of the B-ELISAs with the experimentally produced sera showed the assays to be serovar specific. With the exception of one serum, the serovar A B-ELISA detected antibodies only in the chickens vaccinated with 0083. Similarly, with the exception of one serum, the serovar C B-ELISA detected antibodies only in chose chickens vaccinated with Modesto or those chickens challenged with HP31. Overall, the serovar A B-ELISA had a specificity of 99.7% and a sensitivity of 78.7%, whereas the serovar C B-ELISA had a specificity of 99.8% and a sensitivity of 64.7%.