文献类型： 外文期刊
作者： Zhao, Yujuan 1 ; Duan, Cuicui 1 ; Gao, Lei 1 ; Yu, Xue 1 ; Niu, Chunhua 1 ; Li, Shengyu 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agrofood Technol, Changchun, Peoples R China
关键词： probiotics;mutagenesis;lactobacilli;genome shufFLing;adhesion
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
ISSN： 0916-8451
年卷期： 2017 年 81 卷 1 期
页码：
收录情况： SCI
摘要： Genome shuffling is an important method for rapid improvement in microbial strains for desired phenotypes. In this study, ultraviolet irradiation and nitrosoguanidine were used as mutagens to enhance the adhesion of the wild-type Lactobacillus plantarum C88. Four strains with better property were screened after mutagenesis to develop a library of parent strains for three rounds of genome shuffling. Fusants F3-1, F3-2, F3-3, and F3-4 were screened as the improved strains. The in vivo and in vitro tests results indicated that the population after three rounds of genome shuffling exhibited improved adhesive property. Random Amplified Polymorphic DNA results showed significant differences between the parent strain and recombinant strains at DNA level. These results suggest that the adhesive property of L. plantarum C88 can be significantly improved by genome shuffling. Improvement in the adhesive property of bacterial cells by genome shuffling enhances the colonization of probiotic strains which further benefits to exist probiotic function.
- 相关文献
作者其他论文 更多>>
-
Notoginsenoside R1 Ameliorate High-Fat-Diet and Vitamin D3-Induced Atherosclerosis via Alleviating Inflammatory Response, Inhibiting Endothelial Dysfunction, and Regulating Gut Microbiota
作者：Ma, Liying;Gao, Yansong;Yang, Ge;Zhao, Zijian;Zhao, Yujuan;Li, Shengyu;Ma, Liying;Zhao, Lei;Zhang, Yuhang;Li, Shenhui;Li, Shengyu
关键词：notoginsenoside R1; inflammation; endothelial damage; gut microbiota
-
Conversion of notoginsenoside R1 to 3β,12β-dihydroxydammar-(E)-20(22),24-diene-6-O-β-D-xylopyranosyl-(1→2)-β-D-glucopyranoside by Lactiplantibacillus plantarum S165 enhanced protective effects of LPS-induced intestinal epithelial barrier injury in Caco-2 cells
作者：Wang, Penghui;Gao, Yansong;Yang, Ge;Zhao, Zijian;Li, Shengyu;Wang, Penghui;Zhao, Lei
关键词：notoginsenoside; probiotic; biotransformation; LPS; intestinal injury
-
Genetic variation in the aquaporin TONOPLAST INTRINSIC PROTEIN 4;3 modulates maize cold tolerance
作者：Zeng, Rong;Zhang, Xiaoyan;Li, Minze;Fu, Diyi;Zhang, Zhuo;Gao, Lei;Zhang, Shuaisong;Yang, Xiaohong;Tian, Feng;Yang, Shuhua;Shi, Yiting;Song, Guangshu;Lv, Qingxue;Yang, Xiaohong;Tian, Feng
关键词：cold stress; maize; TIP4;3; natural variation; stomatal movement; reactive oxygen species
-
Transformation of Ginsenosides by Lactiplantibacillus plantarum MB11 Fermentation: Minor Ginsenosides Conversion and Enhancement of Anti-Colorectal Cancer Activity
作者：Shen, Yunjiao;Gao, Yansong;Yang, Ge;Zhao, Zijian;Zhao, Yujuan;Gao, Lei;Li, Shengyu;Shen, Yunjiao;Zhao, Lei
关键词：probiotic; Lactiplantibacillus plantarum; anticancer; ginsenosides; apoptosis; autophagy
-
Increasing minor ginsenosides contents and enhancing neuroprotective effects of total ginsenosides fermented by Lactiplantibacillus plantarum
作者：Yang, Ge;Gao, Yansong;Gao, Lei;Zhao, Zijian;Zhao, Yujuan;Wang, Chao;Li, Shengyu;Li, Shengyu
关键词：Minor ginsenosides; Lactiplantibacillus plantarum; Biotransformation; Neuroprotection; Inflammation; Apoptosis
-
Harnessing the potential of postbiotics derived from Bacillus coagulans: A promising avenue for obesity management via the gut-liver axis
作者：Zhao, Yujuan;Li, Shengyu;Zhao, Zijian;Chen, Long;Ma, Liying;Ning, Chao
关键词：Obesity; Bacillus coagulans; Gut-liver axis; Postbiotics; SCFAs
-
Lacticaseibacillus rhamnosus TF318 prevents depressive behavior in rats by inhibiting HPA-axis hyperactivity and upregulating BDNF expression
作者：Zhao, Yujuan;Kang, You;Zhao, Zijian;Yang, Ge;Gao, Yansong;Gao, Lei;Wang, Chao;Li, Shengyu
关键词：Depression; L. rhamnosus; Microbiota-gut-brain axis; Brain -derived neurotrophic factor
