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Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus

文献类型： 外文期刊

作者： Wang, Yun-Peng 1 ; Wei, Zheng-Yi 2 ; Zhang, Yu-Ying 2 ; Lin, Chun-Jing 2 ; Zhong, Xiao-Fang 2 ; Wang, Yue-Lin 2 ; Ma, 1 ;

作者机构： 1.Jilin Agr Univ, Fac Agron, Changchun 130118, Peoples R China

2.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130033, Peoples R China

3.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2015 年 16 卷 3 期

页码：

收录情况： SCI

摘要： Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 degrees C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast.

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