文献类型： 外文期刊
作者： Jiang, J. 1 ; Yang, B. L. 1 ; Xia, T. 2 ; Yu, S. M. 2 ; Wu, Y. N. 1 ; Jin, H. 1 ; Li, J. R. 3 ;
作者机构： 1.Dalian Nationalities Univ, Coll Environm & Resources, Biol Sci Dept, Dalian, Liaoning, Peoples R China
2.Jilin Acad Agr Sci, Branch Anim Sci, Grassland Inst, Gongzhuling, Jilin, Peoples R China
3.Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA
关键词： Alfalfa;Salt-tolerant germplasms;Genetic diversity;Random amplified polymorphic DNA
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 2015影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 2 期
收录情况： SCI
摘要： Random amplified polymorphic DNA technology was used to analyze the genetic diversity of 25 salt-tolerant alfalfa varieties using 30 different primers. Results showed that the percentage of polymorphic loci between single-plant DNA was 81.52%, and that between mixed DNA of various varieties was 61.65%. Compared to the mixed DNA samples, single-plant DNA samples can better reveal the level of genetic variation among and between alfalfa varieties. The gene differentiation coefficients of 18 Chinese salt-tolerant alfalfa varieties and 7 American salt-tolerant alfalfa varieties were 0.271 and 0.152, respectively, showing that the exchange of genes between Chinese salt-tolerant alfalfa germplasms was more frequent than that of American germplasms. As a topical cross-pollinated plant, the genetic structure of biological populations of alfalfa was directly linked to its breeding system. According to the analysis of genetic distance (GD), 25 varieties can be divided into 9 groups, among which, the GD of Tumu No. 1 and Tumu No. 2 was the shortest (0.148), and the GD of Jieda No. 1 and Tumu was the longest (0.786). The analysis of genetic diversity of salt-tolerant alfalfa germplasms provided a theoretical basis for the creation of an alfalfa salt-tolerant core germplasm repository and for the selection and breeding of new salt-tolerant varieties.
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