CHARACTERIZATION OF BULK SOIL HUMIN AND ITS ALKALINE-SOLUBLE AND ALKALINE-INSOLUBLE FRACTIONS
文献类型： 外文期刊
作者： Li, Cuilan 1 ; Gao, Shuqing 1 ; Gao, Qiang 1 ; Wang, Lichun 2 ; Zhang, Jinjing 1 ;
作者机构： 1.Jilin Agr Univ, Coll Resource & Environm Sci, Changchun 130122, Peoples R China
2.Jilin Acad Agr Sci, Inst Agr Resources & Environm, Changchun, Peoples R China
关键词： soil humic substances;humin fractionation;elemental composition;C-13 NMR
期刊名称：REVISTA BRASILEIRA DE CIENCIA DO SOLO （ 影响因子：1.683； 五年影响因子：1.918 ）
ISSN： 0100-0683
年卷期： 2015 年 39 卷 1 期
页码：
收录情况： SCI
摘要： Humic substances are the major components of soil organic matter. Among the three humic substance components (humic acid, fulvic acid, and humin), humin is the most insoluble in aqueous solution at any pH value and, in turn, the least understood. Humin has poor solubility mainly because it is tightly bonded to inorganic soil colloids. By breaking the linkage between humin and inorganic soil colloids using inorganic or organic solvents, bulk humin can be partially soluble in alkali, enabling a better understanding of the structure and properties of humin. However, the structural relationship between bulk humin and its alkaline-soluble (AS) and alkaline-insoluble (AIS) fractions is still unknown. In this study, we isolated bulk humin from two soils of Northeast China by exhaustive extraction (25 to 28 times) with 0.1 mol L-1 NaOH + 0.1 mol L-1 Na4P2O7, followed by the traditional treatment with 10 % HF-HCl. The isolated bulk humin was then fractionated into AS-humin and AIS-humin by exhaustive extraction (12 to 15 times) with 0.1 mol L-1 NaOH. Elemental analysis and solid-state C-13 cross-polarization magic angle spinning nuclear magnetic resonance (C-13 CPMAS NMR) spectroscopy were used to characterize and compare the chemical structures of bulk humin and its corresponding fractions. The results showed that, regardless of soil types, bulk humin was the most aliphatic and most hydrophobic, AS-humin was the least aliphatic, and AIS-humin was the least alkylated among the three humic components. The results showed that bulk humin and its corresponding AS-humin and AIS-humin fractions are structurally differed from one another, implying that the functions of these humic components in the soil environment differed.
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