文献类型： 外文期刊

作者： Jin Yong-mei ¹ ; Ma Rui ¹ ; Yu Zhi-jing ¹ ; Wang Ling ² ; Jiang Wen-zhu ³ ; Lin Xiu-feng ¹ ;

作者机构： 1.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130124, Peoples R China

2.Jilin Agr Univ, Sch Life Sci, Changchun 130118, Peoples R China

3.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

关键词： insect resistance;Bt gene;cry2A*transgenic rice;japonica rice variety;striped stem borer

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 3 期

页码：

收录情况： SCI

摘要： A synthetic cry2A* gene encoding Bacillus thuringiensis (Bt) delta-endotoxin that resistance to lepidopteran pest was transformed into japonica rice variety Jijing 88, which is the most widely cultivated variety in Jilin Province, Northeast China, by Agrobacterium-mediated transformation. A total of 106 independent transformants overexpressing cry2A* gene driven by ubiquitin (Ubi) promoter was produced. Three single-copy homozygous transgenic lines were finally selected based on the results of PCR analysis, segregation ratio of Basta resistance, and Southern hybridization analyses. RT-PCR and enzyme linked immune sorbent assay (ELISA) revealed that cry2A* transcripts and protein were highly expressed in these lines. The high level of Cry2A* protein expression resulted in high resistance to rice striped stem borer as evidenced by insect feeding bioassays. Our results demonstrate that cry2A* transgenic japonica rice confers resistance to the rice striped stem borer in the laboratory conditions.