Efficient biotransformation for preparation of pharmaceutically active ginsenoside Compound K by Penicillium oxalicum sp 68
文献类型： 外文期刊
作者： Gao, Juan 1 ; Xu, Wenjing 2 ; Fang, Qiang 3 ; Liang, Fei 1 ; Jin, Rutian 1 ; Wu, Di 1 ; Tai, Guihua 1 ; Zhou, Yifa 1 ;
作者机构： 1.NE Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China
2.Jilin Acad Agr Sci, Inst Plant Protect, Changchun 130033, Peoples R China
3.Changchun Univ Technol, Sch Chem & Life Sci, Changchun 130012, Peoples R China
关键词： Ginsenoside;Compound K;Biotransformation;Penicillium oxalicum;Glycosidase
期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）
ISSN： 1590-4261
年卷期： 2013 年 63 卷 1 期
页码：
收录情况： SCI
摘要： Pathogenic fungus Penicillium oxalicum sp. 68 was screened from soil and identified by ITS sequencing. The strain was found to be able to transform protopanaxadiol-type ginsenosides to produce a series of bioactive metabolites. Glycosidase from the culture of P. oxalicum sp. 68 was partially purified with a simple two-step procedure consisting of DEAE-cellulose chromatography and ammonium sulfate precipitation. Bioactive ginsenoside Compound K was prepared selectively and efficiently by biotransformation of ginsenosides Rb1, Rb2, Rc and Rd using the partially purified glycosidase. The optimal conditions for transforming Rb1 into Compound K were pH 4.0, 55 A degrees C and 0.5 mg mL(-1) Rb1. The sole product is Compound K and the maximum yield reached 87.7 % (molar ratio). The transformation pathways of Rb1, Rb2, Rc and Rd are Rb1 -> Rd -> F2 -> Compound K, Rb2 -> CO -> CY -> Compound K, Rc -> Mb -> Mc -> Compound K and Rd -> F2 -> Compound K, respectively. This biotransformation method showed great potential for preparing minor bioactive ginsenosides, especially Compound K, in the pharmaceutical industry because of its high specificity and favorable environmental compatibility.
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