The activity and kinetic parameters of oxidoreductases in phaeozem in response to long-term fertiliser management
文献类型： 外文期刊
作者： Shang, Z. C. 1 ; Zhang, L. L. 1 ; Wu, Z. J. 1 ; Gong, P. 1 ; Li, D. P. 1 ; Zhu, P. 3 ; Gao, H. J. 3 ;
作者机构： 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
2.Shanghai Res Inst Chem Ind, Shanghai 200062, Peoples R China
3.Jilin Acad Agr Sci, Res Ctr Agr Environm & Resources, Changchun 130124, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
关键词： Enzymatic kinetic parameters;dehydrogenase;catalase;phaeozem;fertilisation
期刊名称：JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION （ 影响因子：3.872； 五年影响因子：3.771 ）
ISSN： 0718-9516
年卷期： 2012 年 12 卷 3 期
页码：
收录情况： SCI
摘要： This study describes the effects of balanced versus nutrient-deficient fertilisation on soil nutrient content and selected oxidoreductase activity and kinetic parameters in a long-term (28 years) field experiment conducted using a phaeozem type soil in the Jilin Province of northeast China. As compared to no or unbalanced fertilisation, balanced fertilisation improved the overall chemical fertility of the soil and significantly increased the activities and Vmax values of soil dehydrogenase and catalase. Compared with control (CK), unbalanced fertilisation (with the exception of P deficiency) significantly increased the total carbon content and soil dehydrogenase activity but had less of an effect on the Vmax of the enzyme, whereas the soil catalase activity and its Vmax were less affected under unbalanced fertilisation conditions. The Km value of soil dehydrogenase increased with the application of chemical NPK combined with farmyard manure but decreased under the application of NPK, NP, and PK. The Km value of soil catalase decreased under the application of NK and showed little difference between CK and the other fertilisation treatments. The variations in the activities and kinetic parameters of the enzymes revealed the benefits of long-term balanced fertilisation, particularly the combined application of chemical and organic fertilisers, by improving the chemical and biological fertility of phaeozem. The results also indicated that unbalanced fertilisation with P deficiency (NK) could enhance phaeozem quality, but this effect was limited.
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